Distribution of high mobility group proteins is equivalent ,E and 14/17 and linker histones H1 and H5 on transcribed and non-transcribed regions of chicken erythrocyte chromatin

Quantitative analysis of distribution of chromosomal proteins on single copy DNA sequences has been further developed. Our approach consists of DNA-protein crosslinking within whole cells or isolated nuclei, specific immunoaffinity isolation of crosslinked complexes via protein and identification of...

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Published in:Nucleic acids research 1991-01, Vol.19 (4), p.717-725
Main Authors: Postnikov, Y V, Shick, V V, Belyavsky, A V, Khrapko, K R, Brodolin, K L, Nikolskaya, T A, Mirzabekov, AD
Format: Article
Language:English
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Summary:Quantitative analysis of distribution of chromosomal proteins on single copy DNA sequences has been further developed. Our approach consists of DNA-protein crosslinking within whole cells or isolated nuclei, specific immunoaffinity isolation of crosslinked complexes via protein and identification of crosslinked DNA by hybridisation with single-stranded DNA probes. The present study shows that transcribed chromatin of chicken embryonic erythrocyte beta globin gene is characterized by about 1.5-2.5-fold higher density of HMG 14/17 and 2-fold lower density of H1 and H5 as compared with non-transcribed chromatin of ovalbumin and lysozyme genes, whereas HMG is equivalent ,E proteins were equally distributed between DNA of both transcribed and non-transcribed genes.
ISSN:0305-1048