EMS-induced lincomycin resistance in red pepper (Capsicum annuum L.)

Ethyl methane sulphonate (EMS) is a potential mutagen to induce lincomycin resistance in Capsicum annuum. Mutagenized cotyledons were cultured on shoot regenerating medium containing lincomycin ($100 mgl^{-1}$). Approximately 14% of regenerated shoots were chlorophyll deficient and about 4% of regen...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1997-10, Vol.33 (4), p.285-287
Main Authors: Subhash, K, Venkataiah, P, Bhaskar, P
Format: Article
Language:English
Subjects:
ADN
Agronomy. Soil science and plant productions
ANTIBIOTICOS
ANTIBIOTICS
ANTIBIOTIQUE
APTITUD REGENERATIVA
APTITUDE A LA REGENERATION
Biological and medical sciences
CAPSICUM ANNUUM
CHLOROPHYLLE
CHLOROPHYLLS
CHLOROPLAST DNA
CHLOROPLASTE
CHLOROPLASTS
CLOROFILAS
CLOROPLASTO
COTILEDONES
COTYLEDON
COTYLEDONS
CULTURE MEDIA
CYTOPLASMIC INHERITANCE
DNA
DRUGS
EMS
ETHYL METHANESULFONATE
Fundamental and applied biological sciences. Psychology
GENETIC MARKERS
Genetic mutation
Genetic Transformation/Somatic Cell Genetics
GENETICA
GENETICS
Genetics and breeding of economic plants
GENETIQUE
HEREDITE CYTOPLASMIQUE
HERENCIA CITOPLASMICA
Hybridity
MADRE
MARCADORES GENETICOS
MARQUEUR GENETIQUE
MATERNAL EFFECTS
MEDICAMENT
MEDICAMENTOS
MEDIO DE CULTIVO
MERE
MILIEU DE CULTURE
MOTHERS
MUTACION
MUTAGENESIS
MUTATION
Organelles
Plant breeding: fundamental aspects and methodology
Plant cells
Plants
Plastids
REGENERATIVE ABILITY
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTANCE TO CHEMICALS
RESISTENCIA A PRODUCTOS QUIMICOS
Seedlings
STEMS
TALLO
TIGE
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Summary:Ethyl methane sulphonate (EMS) is a potential mutagen to induce lincomycin resistance in Capsicum annuum. Mutagenized cotyledons were cultured on shoot regenerating medium containing lincomycin ($100 mgl^{-1}$). Approximately 14% of regenerated shoots were chlorophyll deficient and about 4% of regenerated shoots were green from mutaganized cotyledons. The regenerated green plants were resistant to lincomycin but sensitive to chloramphenicol, kanamycin, spectinomycin, and streptomycin. Reciprocal crosses were made between resistant and sensitive plants. Inheritance of lincomycin resistance was transmitted as a non-Mendelian trait. Lincomycin resistance is a first selectable and maternally inherited organelle encoded genetic marker described in chili pepper. Such mutants should be useful in designing biochemical selection schemes to recover somatic hybrids and cybrids.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-997-0051-5