Properties of hybrid complexes composed of photosynthetic reaction centers from the purple bacterium Rhodobacter sphaeroides and quantum dots in lecithin liposomes

Quantum dots (QDs) can absorb ultraviolet and long-wavelength light energy much more efficiently than natural light-harvesting proteins and transfer the excitation energy to photosynthetic reaction centers (RCs). Inclusion into liposomes of RC membrane pigment-protein complexes combined with QDs as...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2014-11, Vol.79 (11), p.1183-1191
Main Authors: Zagidullin, V E, Lukashev, E P, Knox, P P, Seifullina, N Kh, Sokolova, O S, Pechnikova, E V, Lokstein, H, Paschenko, V Z
Format: Article
Language:English
Subjects:
Analysis
Bacteriochlorophylls - chemistry
Cryoelectron microscopy
Electrophoresis
Energy efficiency
Energy transformation
Fluorescence spectroscopy
Lecithin
Lecithins
Liposomes
Liposomes - chemistry
Liposomes - ultrastructure
Methods
Microscopy, Electron, Transmission
Photochemical Processes
Photosynthetic Reaction Center Complex Proteins - metabolism
Photosynthetic reaction centers
Physiological aspects
Production processes
Properties
Proteobacteria
Quantum dots
Quantum Dots - chemistry
Rhodobacter sphaeroides - metabolism
Spectra
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Summary:Quantum dots (QDs) can absorb ultraviolet and long-wavelength light energy much more efficiently than natural light-harvesting proteins and transfer the excitation energy to photosynthetic reaction centers (RCs). Inclusion into liposomes of RC membrane pigment-protein complexes combined with QDs as antennae opens new opportunities for using such hybrid systems as a basis for artificial energy-transforming devices that potentially can operate with greater efficiency and stability than devices based only on biological components. RCs from Rhodobacter sphaeroides and QDs with fluorescence maximum at 530 nm (CdSe/ZnS with hydrophilic covering) were embedded in lecithin liposomes by extrusion of a solution of multilayer lipid vesicles through a polycarbonate membrane or by dialysis of lipids and proteins dispersed with excess detergent. The dimensions of the resulting hybrid systems were evaluated using dynamic light scattering and by transmission cryoelectron microscopy. The efficiency of RC and QD interaction within the liposomes was estimated using fluorescence excitation spectra of the photoactive bacteriochlorophyll of the RCs and by measuring the fluorescence decay kinetics of the QDs. The functional activity of the RCs in hybrid complexes was fully maintained, and their stability was even increased.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297914110054