Measurement of natural rubber latex allergen levels in medical gloves by allergen‐specific IgE‐ELISA inhibition, RAST inhibition, and skin prick test

Exposure to natural rubber latex (NRL) medical gloves poses risks to patients sensitized to NRL and to users of protective gloves. Previous studies have shown that extractable allergen levels of the gloves vary widely. Since most of the available laboratory methods of NRL allergen measurement lack a...

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Bibliographic Details
Published in:Allergy (Copenhagen) 1998-01, Vol.53 (1), p.59-67
Main Authors: Palosuo, T., Mäkinen‐Kiljunen, S., Alenius, H., Reunala, T., Yip, E., Turjanmaa, K.
Format: Article
Language:English
Subjects:
Adolescent
Adult
allergens
Allergens - analysis
Allergens - immunology
Antigens, allergens
Biological and medical sciences
Child
ELISA inhibition
Enzyme-Linked Immunosorbent Assay - methods
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gloves, Surgical - adverse effects
Humans
Hypersensitivity, Immediate - diagnosis
Hypersensitivity, Immediate - immunology
IgE
Immediate hypersensitivity. Allergy. Anaphylaxis, etc
Immunobiology
Immunoglobulin E - analysis
Immunoglobulin E - immunology
latex proteins
Male
medical gloves
natural rubber latex
Proteins - analysis
Proteins - immunology
Radioallergosorbent Test
RAST inhibition
Rubber - adverse effects
Sensitivity and Specificity
skin prick test
Skin Tests
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Summary:Exposure to natural rubber latex (NRL) medical gloves poses risks to patients sensitized to NRL and to users of protective gloves. Previous studies have shown that extractable allergen levels of the gloves vary widely. Since most of the available laboratory methods of NRL allergen measurement lack adequate validation, we wanted to evaluate the performance of a recently developed competitive IgE‐ELISA‐inhibition method in relation to the skin prick test (SPT) and RAST inhibition, as well as to extractable protein quantification and an immunochemical latex antigen assay (LEAP). Twenty samples of surgical (n = l4) and examination gloves (n=6), covering >90% of medical gloves marketed in Finland in 1994–5. were collected by the Finnish National Research and Development Centre for Welfare and Health, coded, extracted, and analyzed by the five methods. The IgE‐ELISA inhibition correlated highly significantly with SPT (r=0.94) and RAST inhibition (r=0.96). Likewise, ELISA inhibition and RAST inhibition showed highly significant correlation (P=0.96, P
ISSN:0105-4538
1398-9995
DOI:10.1111/j.1398-9995.1998.tb03774.x