Importance of CCL25 in the attraction of T cells and the role of IL-7 on the signaling pathways in intestinal epithelial cells

• Published in: Immunobiology (1979) 2008, Vol.214 (6), p.403-409
• Main Authors: Calabrese, Graciela C, Luczak, Erica N, Estela Roux, M
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Allergy and Immunology; Animals; Apoptosis; Bcl-2; CCL25; Chemokines, CC - genetics; Chemokines, CC - metabolism; Chemotaxis, Leukocyte; IL-7; Immunologic Deficiency Syndromes - immunology; Immunologic Deficiency Syndromes - metabolism; Immunologic Deficiency Syndromes - pathology; Interleukin-7 - genetics; Interleukin-7 - metabolism; Intestinal epithelial cells; Intestinal Mucosa - cytology; Intestinal Mucosa - immunology; Intestinal Mucosa - metabolism; Male; Malnutrition - immunology; Malnutrition - metabolism; Malnutrition - pathology; NF-kappa B - metabolism; NF-κB; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rats; Rats, Wistar; Receptors, Antigen, T-Cell, gamma-delta - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Secondary immunodeficiency; Signal Transduction - immunology; Stat3; STAT3 Transcription Factor - metabolism; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism
• ISSN: 0171-2985; 1878-3279
• DOI: 10.1016/j.imbio.2008.12.008

Abstract We have demonstrated an inflammatory process triggered by a dietary antigen accompanied with an increase of TNF-α in an experimental model of secondary immunodeficiency. TNF-α regulates the increase of γδ T subpopulation both in lamina propria and in the intestinal intraepithelium, where IL-7 influences γδ T cells development. TECK (CCL25) is important in the attraction and generation of T and B lymphocytes to the small intestine, lamina propria and intraepithelium. In the present study we evaluate CCL25 and IL-7 mRNA levels, by semiquantitative RT-PCR in intestinal epithelial cells. Lower levels of CCL25 mRNA were detected in animals after re-feeding compared to the well-nourished ones ( P