Human papillomavirus typing by single tube multiplex amplification in real time (SMART): The PapillomaFinder® SMART 20 assay

Highlights • A single-tube multiplex PCR-based HPV detection and typing assay is presented. • The assay can be performed on standard real-time PCR platforms. • All 14 carcinogenic HPV types and 6 low-risk HPV types are detected. • The 10 most important carcinogenic HPV types are recognized separatel...

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Bibliographic Details
Published in:Journal of clinical virology 2014-12, Vol.61 (4), p.540-547
Main Authors: Ossel, J, Litjens, R.J, Reijans, M, Brink, A.A.T.P, Ummelen, M, Ramaekers, F.C, Hopman, A.H.N, Simons, G
Format: Article
Language:English
Subjects:
Allergy and Immunology
Cervical cancer
Female
Genotyping Techniques - methods
Human papillomavirus
Humans
Infectious Disease
Multiplex
Multiplex Polymerase Chain Reaction - methods
Papillomaviridae - classification
Papillomaviridae - genetics
Papillomavirus Infections - diagnosis
Papillomavirus Infections - virology
Real-time polymerase chain reaction
Real-Time Polymerase Chain Reaction - methods
Sensitivity and Specificity
Time Factors
Typing
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Summary:Highlights • A single-tube multiplex PCR-based HPV detection and typing assay is presented. • The assay can be performed on standard real-time PCR platforms. • All 14 carcinogenic HPV types and 6 low-risk HPV types are detected. • The 10 most important carcinogenic HPV types are recognized separately.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2014.10.005