Phosphatidylserine synthesis in glioma C6 cells is inhibited by Ca super(2+) depletion from the endoplasmic reticulum: Effects of 2,5-di-tert-butylhydroquinone and thimerosal

The effects of 2,5-di-terbutylhydroquinone (DBHQ) and thimerosal on phosphatidylserine synthesis by the base exchange reaction and on calcium mobilization in intact glioma C6 cells were compared with that of thapsigargin, a selective inhibitor of the endoplasmic reticulum Ca super(2+)-ATPase. It has...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-07, Vol.224 (3), p.645-650
Main Authors: Wiktorek, M, Sabala, P, Czarny, M, Baranska, J
Format: Article
Language:English
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Summary:The effects of 2,5-di-terbutylhydroquinone (DBHQ) and thimerosal on phosphatidylserine synthesis by the base exchange reaction and on calcium mobilization in intact glioma C6 cells were compared with that of thapsigargin, a selective inhibitor of the endoplasmic reticulum Ca super(2+)-ATPase. It has been found that all these agents inhibit phosphatidylserine synthesis by 70%, but their effectiveness are different. The data show that this inhibition is caused by Ca super(2+) depletion of the endoplasmic reticulum, indicating that phosphatidylserine synthesis requires high concentration of Ca super(2+) within this structure. On this basis and on literature data, a new model for the localization of the serine base exchange enzyme in the endoplasmic reticulum membrane is proposed.
ISSN:0006-291X