Expression and complex assembly of calcium-binding proteins MRP8 and MRP14 during differentiation of murine myelomonocytic cells

In the present study we analyzed expression and biochemical properties of the two recently cloned calcium‐binding proteins MRP8 and MRP14, both members of the S‐100 family, in murine myelomonocytic cells. Expression of MRPs was found to be restricted to granulocytes and distinct stages of macrophage...

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Bibliographic Details
Published in:Journal of leukocyte biology 1993-01, Vol.53 (1), p.11-18
Main Authors: Goebeler, Matthias, Roth, Johannes, Henseleit, Ute, Sunderkotter, Cord, Sorg, Clemens
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Blotting, Western
Bone Marrow - physiology
Bone Marrow Cells
Calcium-Binding Proteins - analysis
Calcium-Binding Proteins - biosynthesis
Calcium-Binding Proteins - metabolism
Calgranulin A
Calgranulin B
Cell Differentiation
Cell Line
Cross-Linking Reagents
Female
granulocytes
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - physiology
inflammation
macrophages
Mice
Mice, Inbred BALB C
monocytes
Monocytes - cytology
Monocytes - physiology
mouse
Organ Specificity
RNA - genetics
RNA - isolation & purification
RNA, Messenger - metabolism
Succinimides
S‐100 protein family
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Summary:In the present study we analyzed expression and biochemical properties of the two recently cloned calcium‐binding proteins MRP8 and MRP14, both members of the S‐100 family, in murine myelomonocytic cells. Expression of MRPs was found to be restricted to granulocytes and distinct stages of macrophage differentiation when compared to the expression of other markers (Mac‐1, F4/80) in transformed macrophage cell lines (Ml, RMB.TG, WEHI.TG, J774A, P388D) and resident and exudate peritoneal and tissue macrophages. Similarly, mRNA and protein levels of MRP8/MRP14 in murine bone marrow cells decreased during culture in L cell‐conditioned medium. Applying a cross‐linking technique, the formation of noncovalently associated heteromeric MRP8/MRP14 complexes of 25, 35, and 48 kd was demonstrated. Our data suggest that MRP8/MRP14 expression and complexation are characteristic for granulocytes and distinct stages of macrophage differentiation in mice. Analysis of the MRP8/MRP14+ phenotype of macrophages may provide further insights into the mechanisms underlying macrophage differentiation.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.53.1.11