Composition of poly-β-hydroxyalkanoate from Syntrophomonas wolfei grown on unsaturated fatty acid substrates

Poly- beta -hydroxyalkanoate (PHA) from crotonate-grown cultures of Syntrophomonas wolfei contained only the D-isomer of beta -hydroxybutyrate. The PHA from cultures grown with trans-2-pentenoate or one of several hexenoates as the substrate also contained small amounts (5%) of beta -hydroxypentanoa...

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Bibliographic Details
Published in:Archives of microbiology 1991, Vol.155 (2), p.103-106
Main Authors: AMOS, D. A, MCINERNEY, M. J
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Growth, nutrition, cell differenciation
Microbiology
Syntrophomonas wolfei
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Summary:Poly- beta -hydroxyalkanoate (PHA) from crotonate-grown cultures of Syntrophomonas wolfei contained only the D-isomer of beta -hydroxybutyrate. The PHA from cultures grown with trans-2-pentenoate or one of several hexenoates as the substrate also contained small amounts (5%) of beta -hydroxypentanoate of beta -hydroxyhexanoate, respectively. Thus, some PHA was synthesized without cleavage of the carbon skeleton of the substrate, but the predominant route for PHA synthesis was by the condensation and subsequent reduction of acetyl-coenzyme A (CoA). The data indicated that the unbroken carbon-chain was used for polymer production only in the early stages of growth and, later, polymer synthesis occurred by the condensation and reduction of acetyl-CoA molecules.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00248601