Characterization and Interfacial Behavior of Nanoparticles Prepared from Amphiphilic Hydrolysates of β‑Conglycinin–Dextran Conjugates

• Published in: Journal of agricultural and food chemistry 2014-12, Vol.62 (52), p.12678-12685
• Main Authors: Wu, Na-Na, Zhang, Jin-Bo, Tan, Bin, He, Xiu-Ting, Yang, Juan, Guo, Jian, Yang, Xiao-Quan
• Format: Article
• Language: English
• Subjects: absorption; adsorption; Antigens, Plant - chemistry; beta-conglycinin; composite polymers; Dextrans - chemistry; Globulins - chemistry; hydrolysates; Hydrolysis; Hydrophobic and Hydrophilic Interactions; hydrophobicity; light scattering; nanoparticles; Nanoparticles - chemistry; oil-water interface; Polymers - chemistry; Seed Storage Proteins - chemistry; Soybean Proteins - chemistry; Surface Properties; transmission electron microscopy; trypsin; Trypsin - chemistry; Water - chemistry; X-radiation
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf504173z

Amphiphilic graft copolymers were prepared from β-conglycinin–dextran conjugates hydrolyzed by trypsin at a degree of hydrolysis (DH) of 2.2%. Nanoparticles were prepared from β-conglycinin, β-conglycinin–dextran conjugates (CDC), and amphiphilic hydrolysates of β-conglycinin–dextran conjugates at DH 2.2% (CDCH) by a desolvation method. All of the nanoparticle samples exhibited spherical structures, as evidenced by dynamic light scattering, transmission electron microscopy, and small-angle X-ray scattering. The nanoparticles prepared from amphiphilic hydrolysates of β-conglycinin–dextran conjugates at DH 2.2% (CDCHN) exhibited higher interfacial pressure and dilatational modulus after long-term absorption at the oil–water interface compared with nanoparticles prepared from β-conglycinin (CN) and β-conglycinin–dextran conjugates (CDCN). This might be mainly associated with the higher surface hydrophobicity of CDCHN, which enhanced adsorption and intermolecular interactions of nanoparticles in the adsorbed layer.