Physical and genetic map of the genome of Staphylococcus carnosus TM300

• Published in: Microbiology (Society for General Microbiology) 1998-02, Vol.144 (2), p.509-517
• Main Authors: Wagner, Elke, Doskar, Jiri, Gotz, Friedrich
• Format: Article
• Language: English
• Subjects: Bacteriology; Biological and medical sciences; Chromosome Mapping; DNA Probes; DNA Restriction Enzymes - metabolism; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field; Food Microbiology; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; Genome, Bacterial; Microbiology; Multigene Family; Nucleic Acid Hybridization; Staphylococcus - genetics; Staphylococcus carnosus
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-144-2-509

Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic ABSTRACT A genome map of Staphylococcus carnosus TM300, an important micro-organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with Not I, Sfi I and Apa I. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the S. carnosus chromosome. Friedrich Götz. Tel: [phone]. Fax: [phone]. e-mail: [e-mail] Keywords: genome mapping, Staphylococcus carnosus