Effects of Flunixin Meglumine and Prostaglandin F2α Treatments on the Development and Quality of Bovine Embryos In Vitro

• Published in: Reproduction in domestic animals 2014-12, Vol.49 (6), p.957-963
• Main Authors: Kim, S‐S, Bang, J‐I, Fakruzzaman, M, Lee, K‐L, Ko, D‐H, Ghanem, N, Wang, Z, Kong, I‐K
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; apoptosis; artificial insemination; Biomarkers; blastocyst; caspase-3; cattle; Cattle - embryology; Clonixin - analogs & derivatives; Clonixin - pharmacology; Culture Media; Dinoprost - pharmacology; DNA, Complementary - genetics; DNA, Complementary - metabolism; Embryo Culture Techniques - veterinary; embryo transfer; Embryo, Mammalian - drug effects; embryogenesis; flunixin; gene expression; Gene Expression Regulation, Developmental - drug effects; Genes, Developmental - physiology; Oxytocics - pharmacology; prostaglandins; quantitative polymerase chain reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; secretion; seminal vesicles; uterus
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/rda.12413

Assisted reproduction procedures, such as embryo transfer (ET) and artificial insemination (AI), in cattle could induce the secretion of prostaglandin F₂‐alpha (PGF₂α) from uterine horns which may in turn interrupt embryo development and implantation. This study investigated the effect of flunixin meglumine (FM), prostaglandin F2 alpha (PGF2α) and FM combined with PGF2α supplementation in culture medium (IVC‐II) on the development and quality of in vitro produced bovine embryos. The development rate of embryos was significantly higher in the FM group (33.3%) than in control (24.3%), PGF₂α (23.9%) and FM + PGF₂α groups (24.5%). The percentage of hatched blastocysts was also higher (p