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Effects of Flunixin Meglumine and Prostaglandin F2α Treatments on the Development and Quality of Bovine Embryos In Vitro
Assisted reproduction procedures, such as embryo transfer (ET) and artificial insemination (AI), in cattle could induce the secretion of prostaglandin F₂‐alpha (PGF₂α) from uterine horns which may in turn interrupt embryo development and implantation. This study investigated the effect of flunixin m...
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| Published in: | Reproduction in domestic animals 2014-12, Vol.49 (6), p.957-963 |
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| container_end_page | 963 |
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| container_title | Reproduction in domestic animals |
| container_volume | 49 |
| creator | Kim, S‐S Bang, J‐I Fakruzzaman, M Lee, K‐L Ko, D‐H Ghanem, N Wang, Z Kong, I‐K |
| description | Assisted reproduction procedures, such as embryo transfer (ET) and artificial insemination (AI), in cattle could induce the secretion of prostaglandin F₂‐alpha (PGF₂α) from uterine horns which may in turn interrupt embryo development and implantation. This study investigated the effect of flunixin meglumine (FM), prostaglandin F2 alpha (PGF2α) and FM combined with PGF2α supplementation in culture medium (IVC‐II) on the development and quality of in vitro produced bovine embryos. The development rate of embryos was significantly higher in the FM group (33.3%) than in control (24.3%), PGF₂α (23.9%) and FM + PGF₂α groups (24.5%). The percentage of hatched blastocysts was also higher (p |
| doi_str_mv | 10.1111/rda.12413 |
| format | article |
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This study investigated the effect of flunixin meglumine (FM), prostaglandin F2 alpha (PGF2α) and FM combined with PGF2α supplementation in culture medium (IVC‐II) on the development and quality of in vitro produced bovine embryos. The development rate of embryos was significantly higher in the FM group (33.3%) than in control (24.3%), PGF₂α (23.9%) and FM + PGF₂α groups (24.5%). The percentage of hatched blastocysts was also higher (p < 0.05) in the FM group (41.2%) than in the control (27.8%) and PGF₂α groups (19.8%). While, there was no significant difference in total cell number in all experimental groups, the number of apoptotic cells was significantly higher in the PGF₂α group (8.2 ± 6.6) than in the control (4.7 ± 3.2), FM (4.7 ± 2.5) and FM + PGF₂α (4.9 ± 3.4) groups. Detected by real‐time PCR, secreted vesicle seminal protein 1 (SSLP1) and prostaglandin G/H synthase 2 (PTGS2) gene expression decreased (p < 0.05) in the PGF₂α group. However, SSLP1 and PTGS2 gene expression in the FM + PGF₂α group returned to their baseline levels, similar to the control and FM groups. Caspase 3 (CAPS3) gene expression increased in the PGF₂α group compared with other groups (p < 0.05). In conclusion, addition of FM in vitro culture significantly improved embryo development as well as alleviated the negative impact of PGF₂α.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12413</identifier><identifier>PMID: 25251522</identifier><language>eng</language><publisher>Germany: P. Parey Scientific Publishers</publisher><subject>Animals ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; apoptosis ; artificial insemination ; Biomarkers ; blastocyst ; caspase-3 ; cattle ; Cattle - embryology ; Clonixin - analogs & derivatives ; Clonixin - pharmacology ; Culture Media ; Dinoprost - pharmacology ; DNA, Complementary - genetics ; DNA, Complementary - metabolism ; Embryo Culture Techniques - veterinary ; embryo transfer ; Embryo, Mammalian - drug effects ; embryogenesis ; flunixin ; gene expression ; Gene Expression Regulation, Developmental - drug effects ; Genes, Developmental - physiology ; Oxytocics - pharmacology ; prostaglandins ; quantitative polymerase chain reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; secretion ; seminal vesicles ; uterus</subject><ispartof>Reproduction in domestic animals, 2014-12, Vol.49 (6), p.957-963</ispartof><rights>2014 Blackwell Verlag GmbH</rights><rights>2014 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25251522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, S‐S</creatorcontrib><creatorcontrib>Bang, J‐I</creatorcontrib><creatorcontrib>Fakruzzaman, M</creatorcontrib><creatorcontrib>Lee, K‐L</creatorcontrib><creatorcontrib>Ko, D‐H</creatorcontrib><creatorcontrib>Ghanem, N</creatorcontrib><creatorcontrib>Wang, Z</creatorcontrib><creatorcontrib>Kong, I‐K</creatorcontrib><title>Effects of Flunixin Meglumine and Prostaglandin F2α Treatments on the Development and Quality of Bovine Embryos In Vitro</title><title>Reproduction in domestic animals</title><addtitle>Reprod Dom Anim</addtitle><description>Assisted reproduction procedures, such as embryo transfer (ET) and artificial insemination (AI), in cattle could induce the secretion of prostaglandin F₂‐alpha (PGF₂α) from uterine horns which may in turn interrupt embryo development and implantation. This study investigated the effect of flunixin meglumine (FM), prostaglandin F2 alpha (PGF2α) and FM combined with PGF2α supplementation in culture medium (IVC‐II) on the development and quality of in vitro produced bovine embryos. The development rate of embryos was significantly higher in the FM group (33.3%) than in control (24.3%), PGF₂α (23.9%) and FM + PGF₂α groups (24.5%). The percentage of hatched blastocysts was also higher (p < 0.05) in the FM group (41.2%) than in the control (27.8%) and PGF₂α groups (19.8%). While, there was no significant difference in total cell number in all experimental groups, the number of apoptotic cells was significantly higher in the PGF₂α group (8.2 ± 6.6) than in the control (4.7 ± 3.2), FM (4.7 ± 2.5) and FM + PGF₂α (4.9 ± 3.4) groups. Detected by real‐time PCR, secreted vesicle seminal protein 1 (SSLP1) and prostaglandin G/H synthase 2 (PTGS2) gene expression decreased (p < 0.05) in the PGF₂α group. However, SSLP1 and PTGS2 gene expression in the FM + PGF₂α group returned to their baseline levels, similar to the control and FM groups. Caspase 3 (CAPS3) gene expression increased in the PGF₂α group compared with other groups (p < 0.05). In conclusion, addition of FM in vitro culture significantly improved embryo development as well as alleviated the negative impact of PGF₂α.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>apoptosis</subject><subject>artificial insemination</subject><subject>Biomarkers</subject><subject>blastocyst</subject><subject>caspase-3</subject><subject>cattle</subject><subject>Cattle - embryology</subject><subject>Clonixin - analogs & derivatives</subject><subject>Clonixin - pharmacology</subject><subject>Culture Media</subject><subject>Dinoprost - pharmacology</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - metabolism</subject><subject>Embryo Culture Techniques - veterinary</subject><subject>embryo transfer</subject><subject>Embryo, Mammalian - drug effects</subject><subject>embryogenesis</subject><subject>flunixin</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Genes, Developmental - physiology</subject><subject>Oxytocics - pharmacology</subject><subject>prostaglandins</subject><subject>quantitative polymerase chain reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>secretion</subject><subject>seminal vesicles</subject><subject>uterus</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNo9kdtSFDEQhlOWlKzohS-gueRmIOfJXCK7C1h4QAEvU9mZzhqdw5rMIPNYvojPRGYX6ZvuSn__X9X5EXpDyRFNdRwqe0SZoPwZmlHBi4xITp-jGSm4ylSu9D56GeNPQqjUef4C7TPJJJWMzdC4cA7KPuLO4WU9tP7et_gjrOuh8S1g21b4S-hib9d1mtNuyf79xdcBbN9AO-la3P8APIc7qLvN9LYVXQ229v042b7v7iarRbMKYxfxRYtvfR-6V2jP2TrC68d-gG6Wi-vT8-zy89nF6cll5hjTPOMlFEWpBehCUFHRCgQhORVMl3kpXbqW5VKtHFOu0CviNLAVWMaJlKoqaM4P0OHOdxO63wPE3jQ-llCne6AboqFKMKo1YUVC3z6iw6qBymyCb2wYzf_vSsDxDvjjaxif9pSYKQeTcjDbHMzX-cl2SIpsp_Cxh_snhQ2_jMp5Ls33T2fmw7mYK3IlzW3i3-14Zztj18FHc_ONpeAIYUwqJfgD2gKSbQ</recordid><startdate>201412</startdate><enddate>201412</enddate><creator>Kim, S‐S</creator><creator>Bang, J‐I</creator><creator>Fakruzzaman, M</creator><creator>Lee, K‐L</creator><creator>Ko, D‐H</creator><creator>Ghanem, N</creator><creator>Wang, Z</creator><creator>Kong, I‐K</creator><general>P. Parey Scientific Publishers</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201412</creationdate><title>Effects of Flunixin Meglumine and Prostaglandin F2α Treatments on the Development and Quality of Bovine Embryos In Vitro</title><author>Kim, S‐S ; Bang, J‐I ; Fakruzzaman, M ; Lee, K‐L ; Ko, D‐H ; Ghanem, N ; Wang, Z ; Kong, I‐K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f2283-3ce99c84e89414d1de40071428c7c5f4392756bf26f98b0f8e2bea230556d9173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>apoptosis</topic><topic>artificial insemination</topic><topic>Biomarkers</topic><topic>blastocyst</topic><topic>caspase-3</topic><topic>cattle</topic><topic>Cattle - embryology</topic><topic>Clonixin - analogs & derivatives</topic><topic>Clonixin - pharmacology</topic><topic>Culture Media</topic><topic>Dinoprost - pharmacology</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - metabolism</topic><topic>Embryo Culture Techniques - veterinary</topic><topic>embryo transfer</topic><topic>Embryo, Mammalian - drug effects</topic><topic>embryogenesis</topic><topic>flunixin</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Genes, Developmental - physiology</topic><topic>Oxytocics - pharmacology</topic><topic>prostaglandins</topic><topic>quantitative polymerase chain reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>secretion</topic><topic>seminal vesicles</topic><topic>uterus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, S‐S</creatorcontrib><creatorcontrib>Bang, J‐I</creatorcontrib><creatorcontrib>Fakruzzaman, M</creatorcontrib><creatorcontrib>Lee, K‐L</creatorcontrib><creatorcontrib>Ko, D‐H</creatorcontrib><creatorcontrib>Ghanem, N</creatorcontrib><creatorcontrib>Wang, Z</creatorcontrib><creatorcontrib>Kong, I‐K</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, S‐S</au><au>Bang, J‐I</au><au>Fakruzzaman, M</au><au>Lee, K‐L</au><au>Ko, D‐H</au><au>Ghanem, N</au><au>Wang, Z</au><au>Kong, I‐K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Flunixin Meglumine and Prostaglandin F2α Treatments on the Development and Quality of Bovine Embryos In Vitro</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Dom Anim</addtitle><date>2014-12</date><risdate>2014</risdate><volume>49</volume><issue>6</issue><spage>957</spage><epage>963</epage><pages>957-963</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Assisted reproduction procedures, such as embryo transfer (ET) and artificial insemination (AI), in cattle could induce the secretion of prostaglandin F₂‐alpha (PGF₂α) from uterine horns which may in turn interrupt embryo development and implantation. This study investigated the effect of flunixin meglumine (FM), prostaglandin F2 alpha (PGF2α) and FM combined with PGF2α supplementation in culture medium (IVC‐II) on the development and quality of in vitro produced bovine embryos. The development rate of embryos was significantly higher in the FM group (33.3%) than in control (24.3%), PGF₂α (23.9%) and FM + PGF₂α groups (24.5%). The percentage of hatched blastocysts was also higher (p < 0.05) in the FM group (41.2%) than in the control (27.8%) and PGF₂α groups (19.8%). While, there was no significant difference in total cell number in all experimental groups, the number of apoptotic cells was significantly higher in the PGF₂α group (8.2 ± 6.6) than in the control (4.7 ± 3.2), FM (4.7 ± 2.5) and FM + PGF₂α (4.9 ± 3.4) groups. Detected by real‐time PCR, secreted vesicle seminal protein 1 (SSLP1) and prostaglandin G/H synthase 2 (PTGS2) gene expression decreased (p < 0.05) in the PGF₂α group. However, SSLP1 and PTGS2 gene expression in the FM + PGF₂α group returned to their baseline levels, similar to the control and FM groups. Caspase 3 (CAPS3) gene expression increased in the PGF₂α group compared with other groups (p < 0.05). In conclusion, addition of FM in vitro culture significantly improved embryo development as well as alleviated the negative impact of PGF₂α.</abstract><cop>Germany</cop><pub>P. Parey Scientific Publishers</pub><pmid>25251522</pmid><doi>10.1111/rda.12413</doi><tpages>7</tpages></addata></record> |
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| ispartof | Reproduction in domestic animals, 2014-12, Vol.49 (6), p.957-963 |
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| subjects | Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology apoptosis artificial insemination Biomarkers blastocyst caspase-3 cattle Cattle - embryology Clonixin - analogs & derivatives Clonixin - pharmacology Culture Media Dinoprost - pharmacology DNA, Complementary - genetics DNA, Complementary - metabolism Embryo Culture Techniques - veterinary embryo transfer Embryo, Mammalian - drug effects embryogenesis flunixin gene expression Gene Expression Regulation, Developmental - drug effects Genes, Developmental - physiology Oxytocics - pharmacology prostaglandins quantitative polymerase chain reaction RNA, Messenger - genetics RNA, Messenger - metabolism secretion seminal vesicles uterus |
| title | Effects of Flunixin Meglumine and Prostaglandin F2α Treatments on the Development and Quality of Bovine Embryos In Vitro |
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