Silver-doped hydroxyapatite coatings formed on Ti–6Al–4V substrates and their characterization

• Published in: Materials Science & Engineering C 2014-03, Vol.36, p.215-220
• Main Authors: Yanovska, A.A., Stanislavov, A.S., Sukhodub, L.B., Kuznetsov, V.N., Illiashenko, V.Yu, Danilchenko, S.N., Sukhodub, L.F.
• Format: Article
• Language: English
• Subjects: Animals; Antibacterial; Bacteria; Bacterial Adhesion - drug effects; Biocompatibility; Chitosan; Chitosan (CS); Chitosan - chemistry; Coated Materials, Biocompatible - pharmacology; Coating; Coatings; Durapatite - pharmacology; Erythrocytes - drug effects; Escherichia coli - drug effects; Escherichia coli - growth & development; Hydroxyapatite; Hydroxyapatite (HA); Microbial Sensitivity Tests; Protective coatings; Sheep; Silver; Silver - pharmacology; Surgical implants; Titanium - pharmacology; Titanium base alloys; X-Ray Diffraction
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2013.12.011

Coatings with antibacterial components for medical implants are recommended to reduce the risk of bacterial infections. Therefore hydroxyapatite (HA) coatings with addition of chitosan (CS) and silver (Ag) are proposed in this work in an attempt to resolve this problem. Ti–6Al–4V substrates were modified by a chitosan film to study the influence of surface modification on the formation of the HA–Ag and HA–CS–Ag coatings. Using a thermal substrate method, HA and HA–CS coatings doped with Ag+ were prepared at low substrate temperatures (90°C). Coated surfaces were examined using X-ray diffraction and scanning electron microscopy. The amount of silver in the deposited coatings was analyzed by atomic absorption spectroscopy. From this study it is concluded that the substrate surface modified by a chitosan film promotes the coating formation and increases the antibacterial activity of produced coatings against a strain of Escherichia coli. The adhesion of E. coli (ATCC 25922) to sheep erythrocytes was decreased by 14% as compared with the reference samples without Ag. It could be explained by the inhibition of bacterial adhesins by Ag+ ions released. The combined action of silver ions and chitosan resulted in a 21% decrease in adhesive index. [Display omitted] •Silver doped hydroxyapatite (HA) coatings are deposited by thermal substrate method.•Surface modification of Ti–6Al–4V substrates by chitosan film is proposed.•The influence of surface modification on HA–Ag coating formation is investigated.•Substrates modified by a chitosan film promote the nucleation of the HA coatings.•Antibacterial effect on the E. coli is more expressed for coatings on modified surface.