Studies on the formation and characterization of macroporous electron-beam generated hyaluronan cryogels

Macroporous cryogels from methacrylated hyaluronan were synthesized at sub-zero temperatures using a novel electron-beam based approach with and without the use of tetra(ethylene glycol) diacrylate as additional cross-linker. Mechanically stable materials possessing a spongy morphology with pore dia...

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Bibliographic Details
Published in:Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2014-12, Vol.105, p.69-77
Main Authors: Reichelt, Senta, Naumov, Sergej, Knolle, Wolfgang, Prager, Andrea, Decker, Ulrich, Becher, Jana, Weisser, Jürgen, Schnabelrauch, Matthias
Format: Article
Language:English
Subjects:
Chain reactions
Computing time
Crosslinking
Cryogels
Electron beam irradiation
Electron paramagnetic resonance
Electron paramagnetic resonance spectroscopy
Formations
Hyaluronan
Nucleation
Porous systems
Quantum chemical calculation
Quantum chemistry
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Summary:Macroporous cryogels from methacrylated hyaluronan were synthesized at sub-zero temperatures using a novel electron-beam based approach with and without the use of tetra(ethylene glycol) diacrylate as additional cross-linker. Mechanically stable materials possessing a spongy morphology with pore diameters ranging from 50µm to 70µm were obtained and characterized by scanning electron microscopy. The excellent cytocompatibility was proven by seeding and cultivation of 3T3 mouse cells on top of the gels. The primary reaction and the chain growth radicals were investigated by quantum chemical calculation and electron paramagnetic resonance spectroscopy and experimentally verified by X-ray photoelectron spectroscopy. The additional cross-linker enhances the reaction efficiency of the cryogel formation. •Successful synthesis of electron-beam derived hyaluronan methacrylate cryogels.•New insight in the reaction mechanism of the formation of hyaluronan-based cryogels.•Enhancement of the reaction efficiency by the addition of a crosslinking agent.•No cytotoxicity was found on the hyaluronan-based cryogels.
ISSN:0969-806X
1879-0895
DOI:10.1016/j.radphyschem.2014.05.021