Effect of sub-acute exposure to nickel nanoparticles on oxidative stress and histopathological changes in Mozambique tilapia, Oreochromis mossambicus

• Published in: Ecotoxicology and environmental safety 2014-09, Vol.107, p.220-228
• Main Authors: Jayaseelan, Chidambaram, Abdul Rahuman, Abdul, Ramkumar, Rajendiran, Perumal, Pachiappan, Rajakumar, Govindasamy, Vishnu Kirthi, Arivarasan, Santhoshkumar, Thirunavukkarasu, Marimuthu, Sampath
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Bundles; Catalase - metabolism; Degeneration; Exposure; Gills - drug effects; Histopathological changes; Liver; Liver - drug effects; Metal Nanoparticles - chemistry; Metal Nanoparticles - toxicity; Muscles; Nickel; Nickel - analysis; Nickel - chemistry; Nickel - toxicity; Nickel nanoparticles; Oreochromis mossambicus; Oxidative stress; Oxidative Stress - drug effects; Superoxide Dismutase - metabolism; Tilapia - metabolism; Transmission electron microscopy; X-Ray Diffraction
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2014.06.012

The aim of the present study was to assess the oxidative stress, antioxidant response and histopathological changes of nickel nanoparticles (Ni NPs) exposure (14 days) in Mozambique tilapia, Oreochromis mossambicus. Ni NPs were synthesized by metal salt reduction method and characterized by X-ray diffraction (XRD) and Transmission electron microscopy (TEM). The XRD peaks at 44°, 51° and 76° were indexed to the (111), (200) and (220) Bragg’s reflections of cubic structure of Nickel, respectively. The crystallite sizes were calculated using Scherrer’s formula applied to the major intense peaks and found to be the size of 56nm. TEM images showed that the synthesized Ni NPs are spherical in shape. Biochemical analysis indicated that the superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity was significantly affected by Ni NPs treated O. mossambicus. Reduced antioxidant enzymes and the contents of antioxidants were lowered in the liver and gills of fishes treated with Ni NPs. After 14 days of exposure, a significant accumulation of Ni in the Ni NPs in experimental group was observed in the gill and skin tissues, with the highest levels found in the liver. Ni NPs exposed fish showed nuclear hypertrophy (NH), nuclear degeneration (ND), necrosis (NC) and irregular-shaped nuclei were observed in liver tissue. The hyperplasia of the gill epithelium (GE), lamellar fusion of secondary lamellae (LF), dilated marginal channel (MC), epithelial lifting (EL) and epithelial rupture were observed in gill tissue. Degeneration in muscle bundles (DM), focal area of necrosis (NC) vacuolar degeneration in muscle bundles (VD), edema between muscle bundles (ED) and splitting of muscle fibers were noticed in skin tissue. Further ecotoxicological evaluation will be made concerning the risk of Ni NPs on aquatic environment. •The toxicity of nickel nanoparticles was examined in Oreochromis mossambicus.•TEM shows Ni NPs are spherical in shape and the crystalline size of 56nm.•Biochemical analysis indicates oxidative stress in tilapia fish caused by Ni NPs.•Accumulation of Ni in the Ni NPs dosed group was observed.•Histopathological alterations were observed in the Ni NPs exposed fishes.