A paper-based calorimetric microfluidics platform for bio-chemical sensing

In this report, a paper-based micro-calorimetric biochemical detection method is presented. Calorimetric detection of biochemical reactions is demonstrated as an extension of current colorimetric and electrochemical detection mechanisms of paper-based biochemical analytical systems. Reaction and/or...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2014-09, Vol.59, p.120-126
Main Authors: Davaji, Benyamin, Lee, Chung Hoon
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
Biotin
Calibration
Calorimetry
Calorimetry - instrumentation
Colorimetry
Deoxyribonucleic acid
Devices
DNA - analysis
Equipment Design
Fundamental and applied biological sciences. Psychology
Glucose
Glucose - analysis
Label-free detection
Limit of Detection
Micro-calorimetry
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Paper-based sensor
Thermal bio-sensor
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Summary:In this report, a paper-based micro-calorimetric biochemical detection method is presented. Calorimetric detection of biochemical reactions is demonstrated as an extension of current colorimetric and electrochemical detection mechanisms of paper-based biochemical analytical systems. Reaction and/or binding temperature of glucose/glucose oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calorimeter. Commercially available glucose calibration samples of 0.05, 0.15 and 0.3% wt/vol concentration are used for comparing the device performance with a commercially available glucose meter (electrochemical detection). The calorimetric glucose detection demonstrates a measurement error less than 2%. The calorimetric detection results of DNA concentrations from 0.9 to 7.3mg/mL and temperature changes in biotin and streptavidin reaction are presented to demonstrate the feasibility of integrating the calorimetric detection method with paper based microfluidic devices. •A thermal (calorimetric) method of the paper-based diagnostic micro-device detects bio-chemical reaction.•Glucose–GOD, DNA–H2O2, and biotin and streptavidin reactions are detected by the paper-based micro-calorimetric device.•Thermal detection of biomaterial at microscale is compared with electrochemical and colorimetric methods.•Bio-chemical concentrations are determined by measuring the temperature change of reactions.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.03.022