Optimization of the enzymatic hydrolysis conditions of steam-exploded wheat straw for maximum glucose and xylose recovery

BACKGROUND: The enzymatic hydrolysis of steam‐exploded wheat straw using commercial enzyme complexes has been studied. A cellulase enzyme complex (Accellerase 1500), along with specific xylanase complements (Accellerase‐XC and Accellerase‐XY) provided by Genencor, have been used to enhance glucose a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2013-02, Vol.88 (2), p.237-246
Main Authors: Marcos, Mónica, García-Cubero, María Teresa, González-Benito, Gerardo, Coca, Mónica, Bolado, Silvia, Lucas, Susana
Format: Article
Language:English
Subjects:
Applied sciences
cellulase
Chemical engineering
Complement
enzymatic hydrolysis
Enzymes
Exact sciences and technology
Glucose
Hydrolysis
Optimization
RSM
Straw
Triticum aestivum
wheat straw
Xylanase
Xylose
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:BACKGROUND: The enzymatic hydrolysis of steam‐exploded wheat straw using commercial enzyme complexes has been studied. A cellulase enzyme complex (Accellerase 1500), along with specific xylanase complements (Accellerase‐XC and Accellerase‐XY) provided by Genencor, have been used to enhance glucose and xylose recovery. A systematic study with response surface methodology (RSM) was used to check the effect of the operating conditions: pH (4–5), temperature (50–60 °C) and enzyme/substrate ratio (0.1–0.5 mL gcellulose−1) on the enzymatic hydrolysis with Acellerase 1500 to maximize the sugar yield. Xylanases were used as complements to increase the release of xylose. RESULT: Statistical results from ANOVA analysis demonstrated that the enzymatic hydrolysis was clearly improved by temperature and enzyme/substrate ratio. The optimum conditions for higher glucose and xylose releases were obtained with the higher enzyme dosage ratio (0.5 mL g−1cellulose), 50 °C and pH 4. CONCLUSION: Model validation at optimum operating conditions showed good agreement between the experimental results and the predicted responses for a confidence level of 95%. The use of the xylanase complements, Accellerase‐XY (accessory xylanase enzyme complex) and Accellerase‐XC (accessory xylanase/cellulase enzyme complex), increases the conversion of hemicellulose. Accellerase‐XC supplementation was more effective, obtaining an increase in yields of glucose and xylose of 11.8% and 23.6%, respectively, using a dosage of 0.125 mL g−1cellulose. © 2012 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.3820