Enzymatic formation of gold nanoparticles by submerged culture of the basidiomycete Lentinus edodes

•Lentinus edodes can reduce Au(III) from HAuCl4 to Au(0), forming nanoparticles.•Au(0) accumulated on the surface and within the hyphae as 5–50nm nanospheres.•The fungal phenol oxidases were found to be involved in the Au reduction. We report for the first time that the medicinal basidiomycete Lenti...

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Bibliographic Details
Published in:Journal of biotechnology 2014-07, Vol.182-183, p.37-45
Main Authors: Vetchinkina, Elena P., Loshchinina, Ekaterina A., Burov, Andrey M., Dykman, Lev A., Nikitina, Valentina E.
Format: Article
Language:English
Subjects:
Basidiomycetes
Cell Culture Techniques - methods
Chlorides - chemistry
Chlorides - metabolism
Enzymes
Extracellular and intracellular synthesis
Forming
Fungal Proteins - metabolism
Fungi
Gold
Gold - chemistry
Gold - metabolism
Gold Compounds - chemistry
Gold Compounds - metabolism
Gold nanoparticles
Lentinus edodes
Light scattering
Metal Nanoparticles - chemistry
Microscopy, Electron, Transmission
Monophenol Monooxygenase - metabolism
Nanoparticles
Particle Size
Phenol oxidases
Reduction
Reduction mechanisms
Shiitake Mushrooms - chemistry
Shiitake Mushrooms - cytology
Shiitake Mushrooms - enzymology
Shiitake Mushrooms - metabolism
Submerged
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Summary:•Lentinus edodes can reduce Au(III) from HAuCl4 to Au(0), forming nanoparticles.•Au(0) accumulated on the surface and within the hyphae as 5–50nm nanospheres.•The fungal phenol oxidases were found to be involved in the Au reduction. We report for the first time that the medicinal basidiomycete Lentinus edodes can reduce Au(III) from chloroauric acid (HAuCl4) to elemental Au [Au(0)], forming nanoparticles. Several methods, including transmission electron microscopy, electron energy loss spectroscopy, X-ray fluorescence, and dynamic light scattering, were used to show that when the fungus was grown submerged, colloidal gold accumulated on the surface of and inside the mycelial hyphae as electron-dense particles mostly spherical in shape, with sizes ranging from 5 to 50nm. Homogeneous proteins (the fungal enzymes laccase, tyrosinase, and Mn-peroxidase) were found for the first time to be involved in the reduction of Au(III) to Au(0) from HAuCl4. A possible mechanism forming Au nanoparticles is discussed.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2014.04.018