Highly selective turn-on fluorescent sensor for nanomolar detection of biologically important Zn2+ based on isonicotinohydrazide derivative: Application in cellular imaging

• Published in: Biosensors & bioelectronics 2014-11, Vol.61, p.429-433
• Main Authors: Tayade, Kundan, Sahoo, Suban K., Bondhopadhyay, Banashree, Bhardwaj, Vimal K., Singh, Narinder, Basu, Anupam, Bendre, Ratnamala, Kuwar, Anil
• Format: Article
• Language: English
• Subjects: Aldehydes - chemistry; Biological and medical sciences; Biosensors; Biotechnology; Cations; Cations, Divalent - analysis; Cellular; Cellular imaging; Crystallography, X-Ray; Derivatives; Fluorescence; Fluorescent Dyes - chemistry; Fluorescent sensor; Fundamental and applied biological sciences. Psychology; HeLa Cells; Humans; Imaging; Isoniazid - analogs & derivatives; Microscopy, Fluorescence - methods; Nanomolar detection; Nanostructure; Optical Imaging - methods; Receptors; Zinc - analysis; Zn2+ detection
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2014.05.053

A new Zn2+ selective chemosensor (3) was synthesized by condensation of commercially available substituted salicylaldehyde and isonicotinohydrazide, and characterized by single crystal X-ray crystallography. Receptor 3 with Zn2+ exhibited a highly selective and pronounced enhancement in the fluorescence emission among different cations by forming a 2:1 complex. The receptor can detect Zn2+ up to nanomolar level (6.75nM) with good tolerance of other metal ions and can be used for in vitro cellular imaging. •Zinc chemosensor was constructed through the selective assembly of a chemosensor.•Fluorescence spectra, a DFT & IR spectrum clearly explains the binding mode between the chemosensor and the detected Zn2+.•The developed sensor was successfully applied to image intracellular Zn2+ in living cells.