The immunotoxicity of graphene oxides and the effect of PVP-coating

Abstract Graphene oxide (GO) immunotoxicity is not clarified well up to date. Herein we reported the effects of GOs with and without polyvinylpyrrolidone (PVP) coating on human immune cells such as dendritic cells (DCs), T lymphocytes and macrophages. Human immune cells such as dendritic cells (DCs)...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2013-07, Vol.34 (21), p.5254-5261
Main Authors: Zhi, Xiao, Fang, Hongliang, Bao, Chenchen, Shen, Guangxia, Zhang, Jiali, Wang, Kan, Guo, Shouwu, Wan, Tao, Cui, Daxiang
Format: Article
Language:English
Subjects:
Advanced Basic Science
Apoptosis - drug effects
Biomedical materials
Blood
Coated Materials, Biocompatible - pharmacology
Cytokines - secretion
Dendritic cells
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - secretion
Dentistry
Graphene
Graphene oxide
Graphite - chemistry
Graphite - toxicity
Human
Humans
Immunotoxicity
Immunotoxins - toxicity
Lymphocytes
Macrophages
Macrophages - cytology
Macrophages - drug effects
Macrophages - metabolism
Microscopy, Atomic Force
Mitochondria - drug effects
Mitochondria - metabolism
Models, Biological
Oxides
Oxides - chemistry
Phenotype
Polyvinylpyrrolidone
Povidone - pharmacology
PVP
T lymphocytes
T-Lymphocytes - cytology
T-Lymphocytes - drug effects
T-Lymphocytes - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Graphene oxide (GO) immunotoxicity is not clarified well up to date. Herein we reported the effects of GOs with and without polyvinylpyrrolidone (PVP) coating on human immune cells such as dendritic cells (DCs), T lymphocytes and macrophages. Human immune cells such as dendritic cells (DCs), T lymphocytes and macrophages were isolated from health donated bloods, PVP-coating GO (PVP-GO) exhibited lower immunogenicity compared with pure GO on the aspect of inducing differentiation and maturation of dendritic cells (DCs), the levels of secreted TNF-α and IL-1β had no obvious difference between two groups, yet the secretion of IL-6 remained in PVP-coating GO group. In addition, PVP-coating GO delayed significantly the apoptotic process of T lymphocytes, at the same time, and exhibited anti-phagocytosis ability against macrophages and markedly enhanced the physiological activity of macrophages. In conclusion, PVP-coating GO possesses good immunological biocompatibility and immunoenhancement effects in vitro , and is likely to be an available candidate of immunoadjuvant in the future.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.03.024