Synergistic effects of dual Zn/Ag ion implantation in osteogenic activity and antibacterial ability of titanium

• Published in: Biomaterials 2014-09, Vol.35 (27), p.7699-7713
• Main Authors: Jin, Guodong, Qin, Hui, Cao, Huiliang, Qian, Shi, Zhao, Yaochao, Peng, Xiaochun, Zhang, Xianlong, Liu, Xuanyong, Chu, Paul K
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Alkaline Phosphatase - metabolism; Animals; Anti-Bacterial Agents - pharmacology; Antiinfectives and antibacterials; Bacteria; Bacterial corrosion; Biocompatibility; Biomedical materials; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Calcification, Physiologic - drug effects; Cells, Cultured; Dentistry; Drug Synergism; Escherichia coli - drug effects; Escherichia coli - ultrastructure; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; Femur - diagnostic imaging; Femur - drug effects; Femur - surgery; Hydrogen-Ion Concentration; Implants, Experimental; Ions; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - enzymology; Mesenchymal Stromal Cells - ultrastructure; Microbial Sensitivity Tests; Models, Biological; Osteogenesis - drug effects; Radiography; Rats; Real-Time Polymerase Chain Reaction; Silver; Silver - pharmacology; Staphylococcus aureus - drug effects; Staphylococcus aureus - ultrastructure; Static Electricity; Surface Properties; Titanium - pharmacology; Titanium base alloys; Zinc; Zinc - pharmacology
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2014.05.074

Abstract Zinc (Zn) and silver (Ag) are co-implanted into titanium by plasma immersion ion implantation. A Zn containing film with Ag nanoparticles (Ag NPs) possessing a wide size distribution is formed on the surface and the corrosion resistance is improved due to the micro-galvanic couples formed by the implanted Zn and Ag. Not only are the initial adhesion, spreading, proliferation and osteogenic differentiation of rBMSCs observed from the Zn/Ag implanted Ti in vitro , but also bacteria killing is achieved both in vitro and in vivo . Electrochemical polarization and ion release measurements suggest that the excellent osteogenic activity and antibacterial ability of the Zn/Ag co-implanted titanium are related to the synergistic effect resulting from the long-range interactions of the released Zn ions and short-range interactions of the embedded Ag NPs. The Zn/Ag co-implanted titanium offers both excellent osteogenic activity and antibacterial ability and has large potential in orthopedic and dental implants.