Antigen detection based on background fluorescence quenching immunochromatographic assay

[Display omitted] •bFQICA is a novel background fluorescence quenching based immunochromatographic assay.•Fluorescence donor in quenching is fluorescein coated on entire nitrocellulose membrane.•bFQICA measurement is based on a unique background vs. specific fluorescence ratio.•bFQICA is exceptional...

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Bibliographic Details
Published in:Analytica chimica acta 2014-09, Vol.841, p.44-50
Main Authors: Chen, Xiangjun, Xu, Yangyang, Yu, Jinsheng, Li, Jiutong, Zhou, Xuelei, Wu, Chuanyong, Ji, Qiuliang, Ren, Yuan, Wang, Liqun, Huang, Zhengyi, Zhuang, Hanling, Piao, Long, Head, Richard, Wang, Yajie, Lou, Jiatao
Format: Article
Language:English
Subjects:
Antigens
Antigens - analysis
Antigens - chemistry
Assaying
Background fluorescence quenching
Biological Assay - methods
Cellulose esters
Fluorescence
Immunochromatography
Membranes
Nitrocellulose
Nitrocellulose membrane
Point of care test
Quantitative detection
Quenching
Time Factors
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Summary:[Display omitted] •bFQICA is a novel background fluorescence quenching based immunochromatographic assay.•Fluorescence donor in quenching is fluorescein coated on entire nitrocellulose membrane.•bFQICA measurement is based on a unique background vs. specific fluorescence ratio.•bFQICA is exceptionally sensitive and of combined qualitative and quantitative capabilities. Gold immunochromatographic assay (GICA) has been around for quite a while, but it is qualitative in the vast majority of applications. A fast, simple and quantitative GICA is in call for better medicine. In the current study, we have established a novel, quantitative GICA based on fluorescence quenching and nitrocellulose membrane background signals, called background fluorescence quenching immunochromatographic assay (bFQICA). Using model analyte alpha-fetoprotein (AFP), the present study assessed the performance of bFQICA in numerous assay aspects. With serial dilutions of the international AFP standard, standard curves for the calculation of AFP concentration were successfully established. At 10 and 100ngmL−1 of the international AFP standard, the assay variability was defined with a coefficient of variance at 10.4% and 15.2%, respectively. For samples with extended range of AFP levels, bFQICA was able to detect AFP at as low as 1ngmL−1. Fluorescence in bFQICA strips stayed constant over months. A good correlation between the results from bFQICA and from a well-established Roche electrochemiluminescence immunoassay was observed in 27 serum samples (r=0.98, p
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2014.07.025