Siderophore mediated uranium sequestration by marine cyanobacterium Synechococcus elongatus BDU 130911

► Uranium induced siderophore production observed in marine cyanobacteria. ► Siderophore – uranium complexation was evidenced by CAS modified assay. ► Siderophore produced by Synechococcus elongatus BDU130911 was identified as hydroxamate type. ► In silico analysis divulges the complexation of sider...

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Bibliographic Details
Published in:Bioresource technology 2013-02, Vol.130, p.204-210
Main Authors: Rashmi, Vijayaraghavan, ShylajaNaciyar, Mohandass, Rajalakshmi, Ramamoorthy, D’Souza, Stanley F., Prabaharan, Dharmar, Uma, Lakshmanan
Format: Article
Language:English
Subjects:
Bio-sequestration
Biological and medical sciences
Complexation
Effectiveness
Elongation
Fundamental and applied biological sciences. Psychology
Hydroxamate
Iron
Marine
Marine cyanobacteria
Molecular Docking Simulation
Organisms
Siderophore
siderophores
Siderophores - biosynthesis
Synechococcus - growth & development
Synechococcus - metabolism
Synechococcus elongatus
tetrazolium
Thin films
Uranium
Uranium - metabolism
Uranium complexation
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Summary:► Uranium induced siderophore production observed in marine cyanobacteria. ► Siderophore – uranium complexation was evidenced by CAS modified assay. ► Siderophore produced by Synechococcus elongatus BDU130911 was identified as hydroxamate type. ► In silico analysis divulges the complexation of siderophore with iron and uranium. Four different marine cyanobacterial morphotypes were tested for their efficacy to produce siderophores in Fe minus [Fe(−)], Fe minus Uranium dosed [Fe(−)U(+)], and Fe dosed Uranium dosed [Fe(−)U(+)] media. Of the four organisms tested, Synechococcus elongatus BDU 130911 produced the highest amount of siderophore of 58μgmg−1 dryweight. The results clearly indicate that uranium induces siderophore production in marine cyanobacteria even in the presence of iron [Fe(−)U(+)] condition. The type of siderophore revealed by FeCl3, Tetrazolium and Atkin’s tests is a hydroxamate; and thin layer chromatogram also authenticates our finding. Uranium siderophore complexation was confirmed through modified Chrome Azurol S (CAS) assay as well as based on residual uranium presence. In silico docking studies further validate siderophore complexation with uranium.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.12.016