A biological assay for the detection of Myrothecium spp. produced macrocyclic trichothecenes

A rapid, inexpensive bioassay to detect Myrothecium spp.-produced macrocyclic trichothecenes was developed. Media containing Myrothecium isolates were inoculated with Chlorella vulgaris, Ustilago maydis and Trichoderma viride. Based on width of the inhibition zone, isolates could be classified as hi...

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Bibliographic Details
Published in:Mycopathologia (1975) 1992-09, Vol.119 (3), p.175-180
Main Authors: BEAN, G. A, JARVIS, B. B, ABOUL-NASR, M. B
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Assay
Chlorella - drug effects
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fundamental and applied biological sciences. Psychology
Microbiology
Mitosporic Fungi - metabolism
Mycology
Myrothecium
Trichoderma - drug effects
Trichothecenes - analysis
Trichothecenes - biosynthesis
Ustilago - drug effects
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Summary:A rapid, inexpensive bioassay to detect Myrothecium spp.-produced macrocyclic trichothecenes was developed. Media containing Myrothecium isolates were inoculated with Chlorella vulgaris, Ustilago maydis and Trichoderma viride. Based on width of the inhibition zone, isolates could be classified as highly toxigenic, non-toxigenic and intermediate. Whereas, C. vulgaris and U. maydis showed significant differences in their response to toxigenic and non-toxigenic isolates, T. viride did not. Production of roridins and verrucarins by the toxigenic isolates (by bioassay) was confirmed by thin layer chromatography and high performance liquid chromatography. This bioassay system, combined with confirmation chemical analyses, increases our ability to detect toxigenic fungal isolates.
ISSN:0301-486X
1573-0832
DOI:10.1007/BF00448816