Cerium oxide-deposited mesoporous silica nanoparticles for the determination of carcinoembryonic antigen in serum using inductively coupled plasma-mass spectrometry

• Published in: Analytica chimica acta 2014-10, Vol.847, p.10-15
• Main Authors: Choi, H.W., Lee, K.H., Hur, N.H., Lim, H.B.
• Format: Article
• Language: English
• Subjects: Antibodies, Immobilized - chemistry; Antigens; Carcinoembryonic Antigen - blood; Cerium - chemistry; Humans; Immunoassay - methods; Limit of Detection; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Mass Spectrometry - methods; Mathematical analysis; Multiplexing; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Serums; Silicon dioxide; Silicon Dioxide - chemistry; Spectrometry; Spectroscopy
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2014.08.041

[Display omitted] •Sandwich-type immunoassay using ICP-MS and nanoparticles to determine biomarkers.•CeO2-deposited mesoporous silica nanoparticles were synthesized as a probe.•Ratiometric measurement significantly improved the calibration linearity.•Excellent detection limit was achieved by signal amplification. CeO2-deposited mesoporous silica nanoparticles were synthesized as a probe to determine carcinoembryonic antigen (CEA) in serum by inductively coupled plasma-mass spectrometry (ICP-MS). The prepared mesoporous nanoparticles were modified and tagged to the target for sandwich-type immunoassay. Fe3O4 magnetic nanoparticles (MNPs) were also synthesized and immobilized with antibody to extract the target biomarker. The calibration curve of the synthesized CeO2-deposited silica nanoparticles, which was plotted by the signal ratio of 140Ce/57Fe measured by ICP-MS vs. the concentration of CEA, showed excellent linearity and sensitivity owing to the signal amplification and low spectral interference. Under optimal conditions, the sandwich-type analytical method was applied to determine CEA in serum spiked in the range of 0.001–5ngmL−1 and showed a limit of detection of 0.36ngmL−1. Since the deposited CeO2 in the mesoporous silica layer can be substituted by other metal compounds, various kinds of metal-deposited nanoparticles can be prepared as probe materials for multiplex detection in bioanalysis.