Deracemization of Amino Acids by Coupling Transaminases of Opposite Stereoselectivity

Biocatalytic deracemization of amino acids without relying on oxidase‐based deamination of an unwanted enantiomer was demonstrated by coupling α‐ and ω‐transaminases displaying opposite stereoselectivity. This strategy employs isopropylamine and a keto acid as cosubstrates and is free of generation...

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Bibliographic Details
Published in:Advanced synthesis & catalysis 2014-11, Vol.356 (17), p.3505-3509
Main Authors: Park, Eul-Soo, Shin, Jong-Shik
Format: Article
Language:English
Subjects:
Amino acids
biocatalysis
Catalysis
coupled enzyme reaction
deracemization
Hydrogen peroxide
Joining
Stereoselectivity
Strategy
Synthesis
Transaminases
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Summary:Biocatalytic deracemization of amino acids without relying on oxidase‐based deamination of an unwanted enantiomer was demonstrated by coupling α‐ and ω‐transaminases displaying opposite stereoselectivity. This strategy employs isopropylamine and a keto acid as cosubstrates and is free of generation of hydrogen peroxide which is troublesome in the conventional oxidase‐based methods.
ISSN:1615-4150
1615-4169
DOI:10.1002/adsc.201400185