Comparative metabolomics analysis on hematopoietic functions of herb pair Gui-Xiong by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry and pattern recognition approach

•Comprehensive metabolomics approach by UHPLC–QTOF/MS coupled with pattern recognition methods.•Hematopoietic functions of herb pair Gui-Xiong.•Thirteen endogenous metabolites were tentatively identified and 12 metabolic pathways were found.•Danggui and Chuanxiong had synergetic hematopoietic effect...

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Published in:Journal of Chromatography A 2014-06, Vol.1346, p.49-56
Main Authors: Li, Weixia, Tang, Yuping, Guo, Jianming, Shang, Erxin, Qian, Yefei, Wang, Linyan, Zhang, Li, Liu, Pei, Su, Shulan, Qian, Dawei, Duan, Jin-ao
Format: Article
Language:English
Subjects:
Angelica
Biological and medical sciences
Chuanxiong
General pharmacology
Hematopoietic function
Herb pair
Herbs
Liquid chromatography
Mass spectrometry
Mathematical models
Medical sciences
Metabolism
Metabolites
Metabolomics
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Quadrupoles
Thiamine
UHPLC–QTOF/MS
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Summary:•Comprehensive metabolomics approach by UHPLC–QTOF/MS coupled with pattern recognition methods.•Hematopoietic functions of herb pair Gui-Xiong.•Thirteen endogenous metabolites were tentatively identified and 12 metabolic pathways were found.•Danggui and Chuanxiong had synergetic hematopoietic effect. The compatibility of Angelicae Sinensis Radix (Danggui, DG) and Chuanxiong Rhizoma (Chuanxiong, CX), a famous herb pair Gui-Xiong (GX), can produce synergistic and complementary hematopoiesis. In present study, global metabolic profiling with ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC–QTOF/MS) combined with pattern recognition method was performed to discover the underlying hematopoietic regulation mechanisms of DG, CX and GX on hemolytic and aplastic anemia rats (HAA) induced by acetyl phenylhydrazine (APH) and cyclophosphamide (CP). Thirteen endogenous metabolites contributing to the separation of model group and control group were tentatively identified. The levels of LPCs including lysoPC (18:0), lysoPC (20:4), lysoPC (16:0) and lysoPC (18:2), sphinganine, nicotinic acid, thiamine pyrophosphate, phytosphingosine, and glycerophosphocholine increased significantly (p
ISSN:0021-9673
DOI:10.1016/j.chroma.2014.04.042