Evaluation of growth, nutrient utilization and production of bioproducts by a wastewater-isolated microalga

• Published in: Bioresource technology 2013-02, Vol.130, p.261-268
• Main Authors: Frampton, Dion M.F., Gurney, Robert H., Dunstan, Graeme A., Clementson, Lesley A., Toifl, Melissa C., Pollard, Chris B., Burn, Stewart, Jameson, Ian D., Blackburn, Susan I.
• Format: Article
• Language: English
• Subjects: Algal Proteins - metabolism; Applied sciences; Arenas; Biodiesel; Biofuel production; Biological and medical sciences; Biomass; Biotechnology; Carbohydrate Metabolism; Cetane; Chlorophyta - growth & development; Chlorophyta - metabolism; Conversion; Energy; Exact sciences and technology; Fatty acids; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Kirchneriella; Microalgae; Microalgae - growth & development; Microalgae - isolation & purification; Microalgae - metabolism; Nutrient uptake; Nutrients; Phosphorus; Pigments; Pigments, Biological - metabolism; Pollution; Receiving; Stress, Physiological; Waste water; Waste Water - microbiology; Wastewaters; Water Microbiology; Water treatment and pollution
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.12.001

► Wastewater isolate, Kirchneriella sp., was grown in laboratory systems up to 60L. ► Successful growth and rapid nutrient uptake in laboratory media and wastewater. ► N-limitation negatively affected FAME yield, positively affected biodiesel quality. ► Promising bioproduct characteristics: FAME, carbohydrate-rich biomass, and pigments. Treatment of wastewater while producing microalgal biomass is receiving ever-increasing attention, particularly in the biofuels arena. In this study, a wastewater chlorophyte isolate, Kirchneriella sp., was tested for its ability to be mass cultivated, utilize nutrients from defined media and wastewater, and produce bioproducts of commercial interest. Growth studies were carried out in various systems at scales up to 60L, with Kirchneriella sp. showing an excellent amenability to being cultured. Biomass concentrations of greater than 1gL−1 were consistently achieved, nitrogen and phosphorus uptake was rapid, and stable medium-term cultures were maintained. Nitrogen limitation affected biomass yield, fatty acid methyl ester (FAME) yield, and cetane index. In contrast, a low phosphorus condition had no effect. Kirchneriella sp. showed an ability to produce several products of commercial value, including carbohydrate-rich biomass, FAME/biodiesel and the pigments β,β-carotene and lutein.