Induction of laccase activity in the white rot fungus Pleurotus ostreatus using water polluted with wheat straw extracts

• Published in: Bioresource technology 2013-04, Vol.133, p.142-149
• Main Authors: Parenti, Alejandra, Muguerza, Elaia, Redin Iroz, Amaia, Omarini, Alejandra, Conde, Enma, Alfaro, Manuel, Castanera, Raúl, Santoyo, Francisco, Ramírez, Lucía, Pisabarro, Antonio G.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Applied sciences; Basidiomycetes; Biological and medical sciences; Biomass; Carbohydrates - analysis; Culture; Effluents; Enzyme Induction - drug effects; Evolution; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; Inducer; Inducers; Isoenzymes - metabolism; Laccase; Laccase - biosynthesis; Laccase - chemistry; Laccase activity; Lignin - metabolism; Liquids; Molecular Sequence Data; Molecular Weight; Phenols - analysis; Pleurotus - drug effects; Pleurotus - enzymology; Pleurotus - growth & development; Pleurotus ostreatus; Pollution; Straw; Triticum - chemistry; Triticum aestivum; Use and upgrading of agricultural and food by-products. Biotechnology; Waste Products - analysis; Wastewater effluent; Wastewaters; Water Pollutants - pharmacology; Water treatment and pollution; Wheat; Wheat straw
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.01.072

[Display omitted] ► Wheat straw water extracts induce efficiently laccase activity in fungal cultures. ► The wheat straw water extract inducer effect is accumulative. ► The isoenzyme Lacc10 is the main responsible for the laccase induced activity. ► Wheat industry polluted water effluents can be used to induce ligninolytic enzymes. The purpose of this work was to explore the use of polluted water effluents from wheat straw using industries as inducers of lignocellulolytic enzymatic activities in cultures of white rot basidiomycetes. For this purpose, we studied the effect of a wheat straw water extract on the evolution of the laccase activity recovered from submerged cultures of Pleurotus ostreatus made in different media and under various culture conditions. Our results demonstrated an accumulative induction effect in all the cultures and conditions tested. This induction is parallel to changes in the laccase electrophoretic profiles recovered from the culture supernatants. The isoenzyme that appeared to be mainly responsible for the laccase activity under these conditions was laccase 10, as confirmed by sequencing the induced protein. These results support the idea of using wheat straw effluents as inducers in liquid cultures of P. ostreatus mycelia for the production of ligninolytic enzymatic cocktails.