Determination of microcystin-LR in water by a label-free aptamer based electrochemical impedance biosensor

In this study, an electrochemical impedance biosensor for cyanobacterial toxin microcystin-LR (MC-LR) detection has been developed. MC-LR aptamers were immobilized on the gold electrode through Au–S interaction, in the presence of target (MC-LR); the binding of MC-LR and aptamers probe led to a comp...

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Bibliographic Details
Published in:Talanta (Oxford) 2013-01, Vol.103, p.371-374
Main Authors: Lin, Zhenyu, Huang, Huiming, Xu, Yixiang, Gao, Xiaoyao, Qiu, Bin, Chen, Xi, Chen, Guonan
Format: Article
Language:English
Subjects:
Aptamer
Aptamers, Nucleotide - metabolism
Binding
Biosensing Techniques
Biosensors
Complex formation
Dielectric Spectroscopy
Electric Impedance
Electrochemical impedance spectroscopy
Electrodes
Gold
Gold - chemistry
Logarithms
Microcystin-LR
Microcystins - analysis
Stability
Toxins
Water - analysis
Water samples
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Summary:In this study, an electrochemical impedance biosensor for cyanobacterial toxin microcystin-LR (MC-LR) detection has been developed. MC-LR aptamers were immobilized on the gold electrode through Au–S interaction, in the presence of target (MC-LR); the binding of MC-LR and aptamers probe led to a complex formation change on the electrode surface and resulted in the impedance decreasing. The decrease rate had a linear relationship with logarithm of the MC-LR concentration in the range of 1.0×10−7–5.0×10−11mol/L, with a detection limit of 1.8×10−11mol/L. The sensor has good selectivity and stability, it has been applied to detect MC-LR in three kinds of real water samples with satisfying results. ► Sensitive and selective biosensor for MC-LR detection has been developed. ► The sensor combined the advantages of aptamer and impedance detection. ► The sensor has been applied to detect MC-LR in real water samples with satisfying results.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2012.10.081