Microbial functioning on crude oil in a gas-permeable single microfluidic channel

Although various studies have been conducted in screening oil-degrading microorganisms and the macroscale degrading efficiency, critical processes of microorganisms in the microscale porous space are not clear, which are important for microbial enhanced oil recovery (MEOR) and bioremediation of oil...

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Bibliographic Details
Published in:Journal of petroleum science & engineering 2013-04, Vol.104, p.38-48
Main Authors: LU WANG, TANG, Yue-Qin, PENG GUO, LUO, Yi-Jing, WU, Xiao-Lei, HAO WANG
Format: Article
Language:English
Subjects:
Applied sciences
Channels
Crude oil
Crude oil, natural gas and petroleum products
Crude oil, natural gas, oil shales producing equipements and methods
Degradation
Dietzia
Droplets
Energy
Enhanced oil recovery
Enhanced oil recovery methods
Exact sciences and technology
Fuels
Microchannels
Microfluidics
Microorganisms
Prospecting and production of crude oil, natural gas, oil shales and tar sands
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Summary:Although various studies have been conducted in screening oil-degrading microorganisms and the macroscale degrading efficiency, critical processes of microorganisms in the microscale porous space are not clear, which are important for microbial enhanced oil recovery (MEOR) and bioremediation of oil contaminated environments. In this work a gas-permeable microchannel filled with crude oil was employed to study the microbial processes in a microscale environment. Cells of a petroleum-degrading Dietzia strain were suspended with two different media and the resulting suspensions were ejected into the microchannel. The degradation processes in the oilasuspension system were then analyzed. The changes in the behavior of the cells and the variation of the oilasuspension interface as well as the evolution of the suspension patterns and structures were observed. aAggregatesa, aclustered cellsa, and aoil dropletsa were characteristically formed in comparison with the control experiments. Oil droplets were formed at the oilasuspension interface and dispersed into the suspension, and this process could be drastic that a plume of oil droplets was issuing into the suspension. All the changes could be attributed to the degradation of crude oil by the cells and the production of biosurfactants which could reduce the interface tension and increase the dissolution of the crude oil. The present work is a preliminary step toward a comprehensive understanding of microbial process in microscale porous space.
ISSN:0920-4105
DOI:10.1016/j.petrol.2013.03.012