Bioreactor performance and functional gene analysis of microbial community in a limited-oxygen fed bioreactor for co-reduction of sulfate and nitrate with high organic input

• Published in: Journal of hazardous materials 2014-08, Vol.278, p.250-257
• Main Authors: Xu, Xi-jun, Chen, Chuan, Wang, Ai-jie, Yu, Hao, Zhou, Xu, Guo, Hong-liang, Yuan, Ye, Lee, Duu-Jong, Zhou, Jizhong, Ren, Nan-qi
• Format: Article
• Language: English
• Subjects: Applied sciences; Bacteria; Bacteria - genetics; Bacteria - metabolism; Beggiatoa; Biological and medical sciences; Bioreactors; Bioreactors - microbiology; Biotechnology; Chemical engineering; Co-reduction; DNA, Bacterial - genetics; Exact sciences and technology; Functional gene; Fundamental and applied biological sciences. Psychology; Genes; Genes, Bacterial; Limited oxygen; Methods. Procedures. Technologies; Microorganisms; Nitrate; Nitrates; Nitrates - metabolism; Others; Oxidation-Reduction; Oxygen - metabolism; Paracoccus denitrificans; Pollution; Reactors; Reduction; Sulfate; Sulfates; Sulfates - metabolism; Sulfides; Thiobacillus denitrificans; Thiomicrospira; Various methods and equipments; Waste Disposal, Fluid - methods; Water Pollutants, Chemical - metabolism
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2014.06.006

•Co-removal of nitrate and sulfate from high organic-laden wastewater was achieved.•Limited-oxygen fed enhanced sulfur recovery, up to 70%.•Functional genes of microbial community were analyzed at limited-oxygen conditions.•Limited oxygen hold strong impact on sulfide-oxidizing genes (fccA/B, sox). Limited-oxygen mediated synergistic relationships between sulfate-reducing bacteria (SRB), nitrate-reducing bacteria (NRB) and sulfide-oxidizing bacteria (SOB, including nitrate-reducing, sulfide-oxidizing bacteria NR-SOB) were predicted to simultaneously remove contaminants of nitrate, sulfate and high COD, and eliminate sulfide generation. A lab-scale experiment was conducted to examine the impact of limited oxygen on these oxy-anions degradation, sulfide oxidation and associated microbial functional responses. In all scenarios tested, the reduction of both nitrate and sulfate was almost complete. When limited-oxygen was fed into bioreactors, S0 formation was significantly improved up to ∼70%. GeoChip 4.0, a functional gene microarray, was used to determine the microbial gene diversity and functional potential for nitrate and sulfate reduction, and sulfide oxidation. The diversity of the microbial community in bioreactors was increased with the feeding of limited oxygen. Whereas the intensities of the functional genes involved in sulfate reduction did not show a significant difference, the abundance of the detected denitrification genes decreased in limited oxygen samples. More importantly, sulfide-oxidizing bacteria may alter their populations/genes in response to limited oxygen potentially to function more effectively in sulfide oxidation, especially to elemental sulfur. The genes fccA/fccB from nitrate-reducing, sulfide-oxidizing bacteria (NR-SOB), such as Paracoccus denitrificans, Thiobacillus denitrificans, Beggiatoa sp., Thiomicrospira sp., and Thioalkalivibrio sp., were more abundant under limited-oxygen condition.