Proteus Mirabilis Bacteria Biosensor Development Based on Modified Gold Electrode with 4-Carboxyphenyl Diazonium Salts for Heavy Metals Toxicity Detection

In this work, the researchers describe a new biosensor for heavy metals detection, based on the immobilization of bacteria, Proteus mirabilis on gold electrode modified with aryl electrografting film. To enhance the stability of the biosystem, additional materials were used such as functionalized Fe...

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Bibliographic Details
Published in:Sensors & transducers 2014-05, Vol.27 (5), p.29-29
Main Authors: Braham, Yosra, Barhoumi, Houcine, Maaref, Abderrazak, Bakhrouf, Amina, Jaffrezic-Renault, Nicole
Format: Article
Language:English
Subjects:
Aqueous solutions
Bacteria
Biosensors
Contact angle
Electrodes
Experiments
Gold
Heavy metals
Immobilization
Metals
Nanoparticles
Potassium
Proteus
Proteus mirabilis
Reagents
Silicon wafers
Spectrum analysis
Toxicity
Voltammetry
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Summary:In this work, the researchers describe a new biosensor for heavy metals detection, based on the immobilization of bacteria, Proteus mirabilis on gold electrode modified with aryl electrografting film. To enhance the stability of the biosystem, additional materials were used such as functionalized Fe sub( 3)O sub( 4) nanoparticles, cationic, anionic polyelectrolytes, Bovine Serum Albumin and glutaraldehyde as a cross-linking agent. Before the immobilization step, the activity of Proteus mirabilis bacteria in the presence of heavy metals ions was attempted using the ion ammonium selective electrodes. The modification of the gold electrodes with the electrochemical reduction of 4-carboxyphenyl diazonium salts to form stable layers for sensing applications was characterized by cyclic voltammetry and chronoamperometry measurements. The immobilized bacteria-metal interaction was evaluated using the electrochemical impedance spectroscopy measurements. A notable effect of metal on the bacteria activity is observed in the concentration range from 10 super( -3) to 1 mu M and from 1 mu M to 1nM for Co super( 2+), Cd super( 2+), Cu super( 2+) and Hg super( 2+), respectively.
ISSN:2306-8515
1726-5479