Isolation of a BTEX-degrading bacterium, Janibacter sp. SB2, from a sea-tidal flat and optimization of biodegradation conditions

► A BTEX-degrading bacterium was isolated from a sea-tidal flat and characterized. ► The isolate, strain SB2, has ability to degrade all six BTEX compounds. ► A mathematical model was employed for the optimization of BTEX degradation. ► Optimum of NH4Cl and NaH2PO4 was determined by response surface...

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Bibliographic Details
Published in:Bioresource technology 2013-10, Vol.145, p.57-64
Main Authors: Jin, Hyun Mi, Choi, Eun Jin, Jeon, Che Ok
Format: Article
Language:English
Subjects:
Actinomycetales - genetics
Actinomycetales - isolation & purification
Actinomycetales - metabolism
Bacteria
Base Sequence
Benzene Derivatives - metabolism
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
BTEX Biodegradation
Cluster Analysis
Culture
Degradation
Denaturing Gradient Gel Electrophoresis
Enrichment
Ethylbenzene
Fundamental and applied biological sciences. Psychology
Geologic Sediments - microbiology
Hydrogen-Ion Concentration
Janibacter sp. SB2
Models, Biological
Molecular Sequence Data
Optimization
Phylogeny
Polynomials
Response surface methodology
RNA, Ribosomal, 16S - genetics
Salinity
Sea water
Sea-tidal flat
Sequence Analysis, DNA
Species Specificity
Strain
Temperature
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Summary:► A BTEX-degrading bacterium was isolated from a sea-tidal flat and characterized. ► The isolate, strain SB2, has ability to degrade all six BTEX compounds. ► A mathematical model was employed for the optimization of BTEX degradation. ► Optimum of NH4Cl and NaH2PO4 was determined by response surface methodology. An enrichment culture was established using seawater containing BTEX (benzene, toluene, ethylbenzene and xylene) compounds to isolate a BTEX-degrading bacterium from contaminated sea-tidal flat. The enriched microbial communities were characterized by 16S rRNA-based DGGE profiling, which indicated that a Janibacter species was dominant during the enrichment. Strain SB2, corresponding to the major band and able to degrade all BTEX compounds, was isolated and characterized. NH4Cl, NaH2PO4, cell mass and BTEX concentrations were used as independent variables to optimize the degradation of BTEX by strain SB2 in a tidal flat and a statistically significant (R2=0.8933, p
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2013.02.004