Investigations on the chromatographic behaviour of zwitterionic stationary phases used in hydrophilic interaction chromatography

Two commercial stationary phases possessing a sulfobetaine zwitterionic bonded ligand (ZIC-HILIC and Nucleodur HILIC) were compared under hydrophilic interaction chromatographic (HILIC) conditions. First of all, the separation of 12 model compounds chosen among neurotransmitters and presenting a div...

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Bibliographic Details
Published in:Journal of Chromatography A 2011-09, Vol.1218 (35), p.5939-5963
Main Authors: Chirita, Raluca-Ioana, West, Caroline, Zubrzycki, Sandrine, Finaru, Adriana-Luminita, Elfakir, Claire
Format: Article
Language:English
Subjects:
acetonitrile
ammonium acetate
Analytical chemistry
Cationic
chemical structure
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
equations
Exact sciences and technology
HILIC
hydrophilic interaction chromatography
hydrophilicity
Ionic interactions
ionization
Ligands
LSER
Mathematical analysis
Mathematical models
Neurotransmitters
Other chromatographic methods
Phases
Retention behaviour
Separation
Solvation parameter model
temperature
Zwitterionic stationary phases
zwitterions
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Summary:Two commercial stationary phases possessing a sulfobetaine zwitterionic bonded ligand (ZIC-HILIC and Nucleodur HILIC) were compared under hydrophilic interaction chromatographic (HILIC) conditions. First of all, the separation of 12 model compounds chosen among neurotransmitters and presenting a diversity of ionization states (anionic, cationic and zwitterionic) was studied under varied operating conditions. The effects of the percentage of acetonitrile, ammonium acetate concentration and temperature of the mobile phase were compared on the two columns. Secondly, a generally applicable retention model was established, based on chromatographic retention data (log k) acquired for 76 model compounds. The chosen compounds are small molecules presenting a wide diversity of molecular structures and are relevant to biomedical and pharmaceutical studies. To account for their retention behaviour, a modified version of the solvation parameter model was designed: two additional molecular descriptors were introduced, to account for ionic interactions with anionic and cationic species. The retention equations obtained allow a rationalization of the interactions contributing to retention and separation in the HILIC systems considered.
ISSN:0021-9673
DOI:10.1016/j.chroma.2011.04.002