A rapid and simplified extraction method of erythromycin from fermentation broth with bond elut C18 cartridge for analysis by HPLC

Solid phase extraction of erythromycin from fermentation broth is described in this paper. This method results in 85 percent recovery of erythromycin A with a batch to batch variability of less than 3 percent. Each column can be used twice with 2-3% loss of recovery. The columns are regenerated imme...

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Bibliographic Details
Published in:Biotechnology techniques 1998-02, Vol.12 (2), p.155-158
Main Authors: HEYDARIAN, S. M, ISON, A. P, MIRJALILI, N
Format: Article
Language:English
Subjects:
Antibiotics
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Production of active biomolecules
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Summary:Solid phase extraction of erythromycin from fermentation broth is described in this paper. This method results in 85 percent recovery of erythromycin A with a batch to batch variability of less than 3 percent. Each column can be used twice with 2-3% loss of recovery. The columns are regenerated immediately after each extraction. In comparison with liquid liquid extraction, solid phase extracts are cleaner, the method is less time consuming and exposure to hazardous solvents is reduced.
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008848719382