Angelicae Gigantis Radix regulates mast cell-mediated allergic inflammation in vivo and in vitro

• Published in: Food and chemical toxicology 2012-09, Vol.50 (9), p.2987-2995
• Main Authors: Sohn, Youngjoo, Lee, Hyun-Sam, Park, Hi-Joon, Lee, Hyangsook, Lee, Hyejung, Choi, Hyeon, Jeong, Chang-Hyun, Bu, Youngmin, Jung, Hyuk-Sang
• Format: Article
• Language: English
• Subjects: Allergy; anaphylaxis; Angelica - chemistry; Angelicae Gigantis Radix; Animals; Biological and medical sciences; calcium; Chromatography, High Pressure Liquid; Cytokine; ethanol; Female; functional foods; Histamine; Histamine Release - drug effects; humans; Hypersensitivity - immunology; Hypersensitivity - prevention & control; immunoglobulin E; In Vitro Techniques; inflammation; Inflammation - immunology; Inflammation - prevention & control; interleukin-8; Jun N-terminal kinase; mast cells; Mast Cells - drug effects; Mast Cells - immunology; Medical sciences; Mice; Mice, Inbred BALB C; Nuclear factor-κB; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; Toxicology; transcription factor NF-kappa B; tumor necrosis factor-alpha
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2012.06.001

► Angelicae gigantis Radix (AG) has anti-allergy effects on in vivo and in vitro models. ► AG reduced chemical-induced PCA and histamine release. ► AG reduced DNFB-induced contact hypersensitivity in mice. ► AG inhibited the production of interleukin (IL)-6, IL-8, and TNF-α in HMC-1 cell. ► AG inhibited the activation of JNK and NF-κB in PMACI-stimulated HMC-1 cell. Angelicae Gigantis (AG) Radix, commonly used medicinal food, has been reported as a promising candidate for inflammatory diseases. However, the anti-allergic effects of AG and its molecular mechanisms have yet to be clarified. The present study investigated the anti-allergy effects of ethanol extracts of AG on mast cell-mediated allergic inflammation in vivo and in vitro. The finding of this study demonstrated that AG reduced anti-dinitrophenyl IgE antibody-induced passive cutaneous anaphylaxis, compound 48/80-induced histamine release, 2,4-dinitrofluoro benzene-induced contact hypersensitivity. In addition, AG inhibited the production of interleukin (IL)-6, IL-8, and TNF-α, as well as the activation of Jun N-terminal kinase and nuclear factor-κB in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated human mast cells. In conclusion, our results provide a novel insight into the pharmacological actions of AG as a potential candidate for use in allergic inflammatory diseases.