Immobilization of Pectinesterase in Genipin-Crosslinked Chitosan Membrane for Low Methoxyl Pectin Production

In this study, an environmentally friendly method for preparation of chitosan crosslinked membranes with different genipin concentrations (0, 125, 250, and 500 mg/L) was developed. Genipin-crosslinked chitosan membranes were used for the immobilization of fungal pectinesterase (PE). PE was efficient...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and biotechnology 2014-12, Vol.174 (8), p.2941-2950
Main Authors: Rangel-Rodríguez, Adriana Marisol, Conxita, Solans, Susana, Vílchez, Flores-Gallardo, Sergio Gabriel, Contreras-Esquivel, Juan Carlos, Licea-Jiménez, Liliana
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Biotechnology
Carboxylic Ester Hydrolases - chemistry
Chemistry
Chemistry and Materials Science
Chitinase
chitosan
Chitosan - chemistry
crosslinking
Enzymes, Immobilized - chemistry
Fourier transform infrared spectroscopy
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungi
General aspects
Immobilization techniques
Infrared spectroscopy
ion exchange chromatography
Iridoids - chemistry
Membranes
Membranes, Artificial
Methods. Procedures. Technologies
pectinesterase
pectins
Pectins - chemistry
Zeta potential
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, an environmentally friendly method for preparation of chitosan crosslinked membranes with different genipin concentrations (0, 125, 250, and 500 mg/L) was developed. Genipin-crosslinked chitosan membranes were used for the immobilization of fungal pectinesterase (PE). PE was efficiently immobilized in chitosan membranes and used for modification of high-methoxylated pectins into low-methoxylated pectins. The charge density (ζ-potential), infrared spectroscopy (FTIR), and ion-exchange chromatography (IEC) revealed the modification of pectin for immobilized PE in genipin-crosslinked chitosan membrane. The ζ-potential results indicated a decrease of −5.2 mV in the pectin control solution, whereas using the PE immobilized in chitosan and genipin-chitosan membranes, the obtained values were −30.45 mV and −36.38 mV, respectively. Genipin-crosslinked chitosan membrane jointly with the immobilized PE represents an eco-friendly support to prepare tailor-made low-methoxylated lime pectin.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-014-1238-y