A Pseudomonas aeruginosa alginate‐exotoxin A conjugate that elicits anti‐alginate and exotoxin A‐neutralizing antibodies

Summary Pseudomonas auruginosa alginate was covalently coupled to exotoxin A by reductive amination using adipic acid dihydrazide as spacer. The conjugate was composed of 25% alginate and 75% exotoxin A and possessed an average molecular mass higher than 700 kDa as determined by polyacrylamide gel e...

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Bibliographic Details
Published in:FEMS microbiology immunology 1991-08, Vol.76 (4), p.185-192
Main Authors: Coin, Dominique, Vacheron, Marie‐Jeanne, Guinand, Micheline, Michel, Georges
Format: Article
Language:English
Subjects:
ADP Ribose Transferases
Alginate
Alginates - isolation & purification
Alginates - pharmacology
Animals
Antibodies, Bacterial - analysis
antibody response
Bacterial Toxins - immunology
conjugate
Exotoxin A
Exotoxins - immunology
Exotoxins - isolation & purification
Exotoxins - pharmacology
immunostimulation
man
Mice
Neutralization Tests
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas aeruginosa Exotoxin A
Rabbits
Virulence Factors
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Summary:Summary Pseudomonas auruginosa alginate was covalently coupled to exotoxin A by reductive amination using adipic acid dihydrazide as spacer. The conjugate was composed of 25% alginate and 75% exotoxin A and possessed an average molecular mass higher than 700 kDa as determined by polyacrylamide gel electrophoresis. The conjugate had virtually no ADP‐ribosyltransferase activity and a reduced cytotoxity for TSA8 murine cells, derived from Friend erythroleukemia cells, as indicated by a > 50‐fold increased LD50. Anti‐conjugate antibodies recognized exotoxin A and alginate. A booster injection resulted in markedly increased antibody ELISA titers to both exotoxin A and alginate. The antibodies neutralized the exotoxin A toxicity.
ISSN:0378-1097
0920-8534
1574-6968
DOI:10.1111/j.1574-6968.1991.tb04213.x