Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum

Five cDNA clones encoding the catalytic subunits of Ser/Thr protein phosphatases (PP) of Nicotiana tabacum were described. Among them, three clones (NPP1, NPP2, and NPP3) encoded type 1 PP (PP1), whereas the rest of the clones (NPP4 and NPP5) encoded type 2A PP (PP2A). These cDNA clones exhibited hi...

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Bibliographic Details
Published in:Plant molecular biology 1998-01, Vol.36 (2), p.315-322
Main Authors: Suh, M C, Cho, H S, Kim, Y S, Liu, J R, Lee, H S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Conserved Sequence
DNA Primers
EXPRESION GENICA
EXPRESSION DES GENES
Flowers
GENE EXPRESSION
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genes
Genes, Plant
Isoenzymes - biosynthesis
Isoenzymes - chemistry
Isoenzymes - genetics
Macromolecular Substances
MOLECULAR CLONING
Molecular Sequence Data
Multigene Family
Nicotiana - enzymology
Nicotiana - genetics
NICOTIANA TABACUM
Phosphoprotein Phosphatases - biosynthesis
Phosphoprotein Phosphatases - chemistry
Phosphoprotein Phosphatases - genetics
Plants, Toxic
Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
SERINA
SERINE
THREONINE
TREONINA
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Description
Summary:Five cDNA clones encoding the catalytic subunits of Ser/Thr protein phosphatases (PP) of Nicotiana tabacum were described. Among them, three clones (NPP1, NPP2, and NPP3) encoded type 1 PP (PP1), whereas the rest of the clones (NPP4 and NPP5) encoded type 2A PP (PP2A). These cDNA clones exhibited high sequence identity in the PP core region to the corresponding genes from animals and plants. NPP1 mRNA was predominantly expressed in flowers, whereas NPP5 mRNA was mainly detected in leaves and flowers. In contrast, the transcripts of NPP2, NPP3, and NPP4 genes were present in all tissues examined. Throughout flower development, NPP1, NPP2, NPP5 mRNAs were expressed without any significant variation at the steady-state level. Genomic Southern blot showed that tobacco genome contained multiple genes and/or pseudogenes for both type 1 and type 2A phosphatases.
ISSN:0167-4412
1573-5028
DOI:10.1023/A:1005943805988