Loading…
Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases
Background and Objective Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long‐term release of reactive oxygen species. A high number of reacti...
Saved in:
| Published in: | Journal of periodontal research 2015-02, Vol.50 (1), p.28-36 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c4335-4d7596a127cbe498bcae038346714b3c44afd4168c892f3b6682789f50e36d8e3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c4335-4d7596a127cbe498bcae038346714b3c44afd4168c892f3b6682789f50e36d8e3 |
| container_end_page | 36 |
| container_issue | 1 |
| container_start_page | 28 |
| container_title | Journal of periodontal research |
| container_volume | 50 |
| creator | Zamora-Perez, A. L. Ortiz-García, Y. M. Lazalde-Ramos, B. P. Guerrero-Velázquez, C. Gómez-Meda, B. C. Ramírez-Aguilar, M. Á. Zúñiga-González, G. M. |
| description | Background and Objective
Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long‐term release of reactive oxygen species. A high number of reactive oxygen species result in periodontal tissue damage through multiple mechanisms such as lipid peroxidation, protein denaturation and DNA damage. The aim of this study was to evaluate DNA and oxidative damage in subjects with chronic or aggressive periodontitis and healthy controls.
Material and Methods
Buccal mucosa cells and whole saliva were collected from 160 subjects, who were divided into three groups: subjects with chronic periodontitis (CP) (n = 58), subjects with aggressive periodontitis (AgP) (n = 42) and a control group (n = 60). DNA damage was determined by counting micronuclei (MN) and nuclear abnormalities (NAs) in exfoliated cells, including binucleated cells, cells with nuclear buds and karyolitic, karyorrhectic, condensed chromatin and pyknotic cells. The degree of oxidative stress was determined by quantifying 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in whole saliva.
Results
Subjects with CP or AgP presented significantly more ( p |
| doi_str_mv | 10.1111/jre.12175 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1643147574</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1643147574</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4335-4d7596a127cbe498bcae038346714b3c44afd4168c892f3b6682789f50e36d8e3</originalsourceid><addsrcrecordid>eNp1kc9u1DAQxi0EotvCgRdAPsIhrR07dnKEqi1FFZXoIo7WxJ5sXZJ4sZP-eRmeFe9u21vn4rH8-76x5iPkA2eHPNfRTcRDXnJdvSILrhgrmFbVa7JgrCwLIWu5R_ZTumH5rnTzluyVUiklVL0g_85HGxESOjp4G8M42x49hdHRbQuRQjuGOEDvJ4-J-pG2s7XQ02G2IcEWDffeweRvkToYYIUbKmXFLdAuhoGu8yOOU6J3frqm9jrP8XarhNUqYkob6RqjDy6MU_Z2Pm0-ld6RNx30Cd8_ngfk1-nJ8vhbcXF5dn785aKwUoiqkE5XjQJeatuibOrWAjJRC6k0l62wUkLnJFe1rZuyE61SdanrpqsYCuVqFAfk0853HcPfGdNkBp8s9j2MGOZkuJKCS11pmdHPOzRvK6WInVlHP0B8MJyZTRwmx2G2cWT246Pt3A7onsmn_WfgaAfc-R4fXnYy33-ePFkWO4VPE94_KyD-MUqLTP7-cWaW5VLJq69Xhon_yWCmfw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1643147574</pqid></control><display><type>article</type><title>Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases</title><source>Wiley</source><creator>Zamora-Perez, A. L. ; Ortiz-García, Y. M. ; Lazalde-Ramos, B. P. ; Guerrero-Velázquez, C. ; Gómez-Meda, B. C. ; Ramírez-Aguilar, M. Á. ; Zúñiga-González, G. M.</creator><creatorcontrib>Zamora-Perez, A. L. ; Ortiz-García, Y. M. ; Lazalde-Ramos, B. P. ; Guerrero-Velázquez, C. ; Gómez-Meda, B. C. ; Ramírez-Aguilar, M. Á. ; Zúñiga-González, G. M.</creatorcontrib><description>Background and Objective
Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long‐term release of reactive oxygen species. A high number of reactive oxygen species result in periodontal tissue damage through multiple mechanisms such as lipid peroxidation, protein denaturation and DNA damage. The aim of this study was to evaluate DNA and oxidative damage in subjects with chronic or aggressive periodontitis and healthy controls.
Material and Methods
Buccal mucosa cells and whole saliva were collected from 160 subjects, who were divided into three groups: subjects with chronic periodontitis (CP) (n = 58), subjects with aggressive periodontitis (AgP) (n = 42) and a control group (n = 60). DNA damage was determined by counting micronuclei (MN) and nuclear abnormalities (NAs) in exfoliated cells, including binucleated cells, cells with nuclear buds and karyolitic, karyorrhectic, condensed chromatin and pyknotic cells. The degree of oxidative stress was determined by quantifying 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in whole saliva.
Results
Subjects with CP or AgP presented significantly more ( p < 0.05) MN and NAs and higher levels of 8‐OHdG ( p < 0.05) compared with the control group.
Conclusion
Our results indicate that subjects with periodontitis (CP or AgP) exhibited an increase in the frequency of MN, NAs and 8‐OHdG, which is directly related to DNA damage. In addition, a positive correlation exists between oxidative stress produced by periodontitis disease and MN.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/jre.12175</identifier><identifier>PMID: 24666368</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adult ; Aggressive Periodontitis - pathology ; Cell Nucleus - pathology ; Chromatin - ultrastructure ; Chronic Periodontitis - pathology ; cytotoxicity ; Dental Plaque Index ; Dentistry ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - analysis ; DNA Damage ; Female ; Humans ; Male ; Micronuclei, Chromosome-Defective ; Middle Aged ; Mouth Mucosa - pathology ; oxidative stress ; Oxidative Stress - physiology ; Periodontal Attachment Loss - classification ; periodontal disease ; Periodontal Index ; Periodontal Pocket - classification ; Saliva - metabolism</subject><ispartof>Journal of periodontal research, 2015-02, Vol.50 (1), p.28-36</ispartof><rights>2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4335-4d7596a127cbe498bcae038346714b3c44afd4168c892f3b6682789f50e36d8e3</citedby><cites>FETCH-LOGICAL-c4335-4d7596a127cbe498bcae038346714b3c44afd4168c892f3b6682789f50e36d8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24666368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zamora-Perez, A. L.</creatorcontrib><creatorcontrib>Ortiz-García, Y. M.</creatorcontrib><creatorcontrib>Lazalde-Ramos, B. P.</creatorcontrib><creatorcontrib>Guerrero-Velázquez, C.</creatorcontrib><creatorcontrib>Gómez-Meda, B. C.</creatorcontrib><creatorcontrib>Ramírez-Aguilar, M. Á.</creatorcontrib><creatorcontrib>Zúñiga-González, G. M.</creatorcontrib><title>Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases</title><title>Journal of periodontal research</title><addtitle>J Periodont Res</addtitle><description>Background and Objective
Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long‐term release of reactive oxygen species. A high number of reactive oxygen species result in periodontal tissue damage through multiple mechanisms such as lipid peroxidation, protein denaturation and DNA damage. The aim of this study was to evaluate DNA and oxidative damage in subjects with chronic or aggressive periodontitis and healthy controls.
Material and Methods
Buccal mucosa cells and whole saliva were collected from 160 subjects, who were divided into three groups: subjects with chronic periodontitis (CP) (n = 58), subjects with aggressive periodontitis (AgP) (n = 42) and a control group (n = 60). DNA damage was determined by counting micronuclei (MN) and nuclear abnormalities (NAs) in exfoliated cells, including binucleated cells, cells with nuclear buds and karyolitic, karyorrhectic, condensed chromatin and pyknotic cells. The degree of oxidative stress was determined by quantifying 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in whole saliva.
Results
Subjects with CP or AgP presented significantly more ( p < 0.05) MN and NAs and higher levels of 8‐OHdG ( p < 0.05) compared with the control group.
Conclusion
Our results indicate that subjects with periodontitis (CP or AgP) exhibited an increase in the frequency of MN, NAs and 8‐OHdG, which is directly related to DNA damage. In addition, a positive correlation exists between oxidative stress produced by periodontitis disease and MN.</description><subject>Adult</subject><subject>Aggressive Periodontitis - pathology</subject><subject>Cell Nucleus - pathology</subject><subject>Chromatin - ultrastructure</subject><subject>Chronic Periodontitis - pathology</subject><subject>cytotoxicity</subject><subject>Dental Plaque Index</subject><subject>Dentistry</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - analysis</subject><subject>DNA Damage</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Micronuclei, Chromosome-Defective</subject><subject>Middle Aged</subject><subject>Mouth Mucosa - pathology</subject><subject>oxidative stress</subject><subject>Oxidative Stress - physiology</subject><subject>Periodontal Attachment Loss - classification</subject><subject>periodontal disease</subject><subject>Periodontal Index</subject><subject>Periodontal Pocket - classification</subject><subject>Saliva - metabolism</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp1kc9u1DAQxi0EotvCgRdAPsIhrR07dnKEqi1FFZXoIo7WxJ5sXZJ4sZP-eRmeFe9u21vn4rH8-76x5iPkA2eHPNfRTcRDXnJdvSILrhgrmFbVa7JgrCwLIWu5R_ZTumH5rnTzluyVUiklVL0g_85HGxESOjp4G8M42x49hdHRbQuRQjuGOEDvJ4-J-pG2s7XQ02G2IcEWDffeweRvkToYYIUbKmXFLdAuhoGu8yOOU6J3frqm9jrP8XarhNUqYkob6RqjDy6MU_Z2Pm0-ld6RNx30Cd8_ngfk1-nJ8vhbcXF5dn785aKwUoiqkE5XjQJeatuibOrWAjJRC6k0l62wUkLnJFe1rZuyE61SdanrpqsYCuVqFAfk0853HcPfGdNkBp8s9j2MGOZkuJKCS11pmdHPOzRvK6WInVlHP0B8MJyZTRwmx2G2cWT246Pt3A7onsmn_WfgaAfc-R4fXnYy33-ePFkWO4VPE94_KyD-MUqLTP7-cWaW5VLJq69Xhon_yWCmfw</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Zamora-Perez, A. L.</creator><creator>Ortiz-García, Y. M.</creator><creator>Lazalde-Ramos, B. P.</creator><creator>Guerrero-Velázquez, C.</creator><creator>Gómez-Meda, B. C.</creator><creator>Ramírez-Aguilar, M. Á.</creator><creator>Zúñiga-González, G. M.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201502</creationdate><title>Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases</title><author>Zamora-Perez, A. L. ; Ortiz-García, Y. M. ; Lazalde-Ramos, B. P. ; Guerrero-Velázquez, C. ; Gómez-Meda, B. C. ; Ramírez-Aguilar, M. Á. ; Zúñiga-González, G. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4335-4d7596a127cbe498bcae038346714b3c44afd4168c892f3b6682789f50e36d8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aggressive Periodontitis - pathology</topic><topic>Cell Nucleus - pathology</topic><topic>Chromatin - ultrastructure</topic><topic>Chronic Periodontitis - pathology</topic><topic>cytotoxicity</topic><topic>Dental Plaque Index</topic><topic>Dentistry</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - analysis</topic><topic>DNA Damage</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Micronuclei, Chromosome-Defective</topic><topic>Middle Aged</topic><topic>Mouth Mucosa - pathology</topic><topic>oxidative stress</topic><topic>Oxidative Stress - physiology</topic><topic>Periodontal Attachment Loss - classification</topic><topic>periodontal disease</topic><topic>Periodontal Index</topic><topic>Periodontal Pocket - classification</topic><topic>Saliva - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zamora-Perez, A. L.</creatorcontrib><creatorcontrib>Ortiz-García, Y. M.</creatorcontrib><creatorcontrib>Lazalde-Ramos, B. P.</creatorcontrib><creatorcontrib>Guerrero-Velázquez, C.</creatorcontrib><creatorcontrib>Gómez-Meda, B. C.</creatorcontrib><creatorcontrib>Ramírez-Aguilar, M. Á.</creatorcontrib><creatorcontrib>Zúñiga-González, G. M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zamora-Perez, A. L.</au><au>Ortiz-García, Y. M.</au><au>Lazalde-Ramos, B. P.</au><au>Guerrero-Velázquez, C.</au><au>Gómez-Meda, B. C.</au><au>Ramírez-Aguilar, M. Á.</au><au>Zúñiga-González, G. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodont Res</addtitle><date>2015-02</date><risdate>2015</risdate><volume>50</volume><issue>1</issue><spage>28</spage><epage>36</epage><pages>28-36</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objective
Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long‐term release of reactive oxygen species. A high number of reactive oxygen species result in periodontal tissue damage through multiple mechanisms such as lipid peroxidation, protein denaturation and DNA damage. The aim of this study was to evaluate DNA and oxidative damage in subjects with chronic or aggressive periodontitis and healthy controls.
Material and Methods
Buccal mucosa cells and whole saliva were collected from 160 subjects, who were divided into three groups: subjects with chronic periodontitis (CP) (n = 58), subjects with aggressive periodontitis (AgP) (n = 42) and a control group (n = 60). DNA damage was determined by counting micronuclei (MN) and nuclear abnormalities (NAs) in exfoliated cells, including binucleated cells, cells with nuclear buds and karyolitic, karyorrhectic, condensed chromatin and pyknotic cells. The degree of oxidative stress was determined by quantifying 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in whole saliva.
Results
Subjects with CP or AgP presented significantly more ( p < 0.05) MN and NAs and higher levels of 8‐OHdG ( p < 0.05) compared with the control group.
Conclusion
Our results indicate that subjects with periodontitis (CP or AgP) exhibited an increase in the frequency of MN, NAs and 8‐OHdG, which is directly related to DNA damage. In addition, a positive correlation exists between oxidative stress produced by periodontitis disease and MN.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24666368</pmid><doi>10.1111/jre.12175</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3484 |
| ispartof | Journal of periodontal research, 2015-02, Vol.50 (1), p.28-36 |
| issn | 0022-3484 1600-0765 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1643147574 |
| source | Wiley |
| subjects | Adult Aggressive Periodontitis - pathology Cell Nucleus - pathology Chromatin - ultrastructure Chronic Periodontitis - pathology cytotoxicity Dental Plaque Index Dentistry Deoxyguanosine - analogs & derivatives Deoxyguanosine - analysis DNA Damage Female Humans Male Micronuclei, Chromosome-Defective Middle Aged Mouth Mucosa - pathology oxidative stress Oxidative Stress - physiology Periodontal Attachment Loss - classification periodontal disease Periodontal Index Periodontal Pocket - classification Saliva - metabolism |
| title | Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T10%3A29%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20micronuclei%20and%20nuclear%20abnormalities%20in%20buccal%20mucosa%20and%20oxidative%20damage%20in%20saliva%20from%20patients%20with%20chronic%20and%20aggressive%20periodontal%20diseases&rft.jtitle=Journal%20of%20periodontal%20research&rft.au=Zamora-Perez,%20A.%20L.&rft.date=2015-02&rft.volume=50&rft.issue=1&rft.spage=28&rft.epage=36&rft.pages=28-36&rft.issn=0022-3484&rft.eissn=1600-0765&rft_id=info:doi/10.1111/jre.12175&rft_dat=%3Cproquest_cross%3E1643147574%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4335-4d7596a127cbe498bcae038346714b3c44afd4168c892f3b6682789f50e36d8e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1643147574&rft_id=info:pmid/24666368&rfr_iscdi=true |