Impact of the stringency of cell selection on plastid segregation in protoplast fusion-derived Nicotiana regenerates

Vegetative segregation of a mixed plastid population in protoplast fusion-derived cell lines can be directed by a selection favouring the multiplication of one of the parental plastid types. This report defines some of the critical conditions leading to a homogeneous plastid population in cybrid pla...

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Bibliographic Details
Published in:Theoretical and applied genetics 1992-09, Vol.84 (7-8), p.866-873
Main Authors: Malone, R. (Hungarian Academy of Sciences, Szeged (Hungary). Biological Research Centre), Horvath, G.V, Cseploe, A, Buzas, B, Dix, P.J, Medgyesy, P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
FUSION DE PROTOPLASTES
FUSION DEL PROTOPLASTO
GENETIC MARKERS
Genetics of eukaryotes. Biological and molecular evolution
MARCADORES GENETICOS
MARQUEUR GENETIQUE
NICOTIANA
Nicotiana plumbaginifolia
Nicotiana tabacum
PLASTE
Plastid genetic markers
Plastid segregation
Plastid selection
PLASTIDIOS
PLASTIDS
PROTOPLAST FUSION
Pteridophyta, spermatophyta
SELECCION
SELECTION
Vegetals
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Summary:Vegetative segregation of a mixed plastid population in protoplast fusion-derived cell lines can be directed by a selection favouring the multiplication of one of the parental plastid types. This report defines some of the critical conditions leading to a homogeneous plastid population in cybrid plants generated by protoplast fusion between Nicotiana plumbaginifolia and an albino and streptomycin-resistant N. tabacum plastid mutant. Light (1,500 lx) conferred a strong selective advantage to chloroplasts versus albino plastids, while the lack of this effect in dim light (300 lx) indicated that a sufficient light intensity is essential to the phenomenon. Selection on streptomycin-containing medium in the dark, however, led to the preferential multiplication of resistant plastids. Streptomycin selection of resistant chloroplasts in the light, consequently, results in a plastid selection of doubled stringency. In another experiment a definite, but leaky, selection for chloroplast recombination (selection for greening on streptomycin-containing medium in dim light) was used to reveal various recombination products. Protoplast fusion in fact resulted in cybrid plants showing only simple chimeric segregation of unchanged parental plastids. These results demonstrate the essential requirement for stringent plastid selection, as defined by cell culture conditions, to precede the formation of shoots expected to possess the desired plastid genetic composition.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00227398