An essential and specific subunit of RNA polymerase III (C) is encoded by gene RPC34 in Saccharomyces cerevisiae

The RPC34 gene of Saccharomyces cerevisiae was cloned by immunological screening, using antibodies raised against the C34 polypeptide of the RNA polymerase III (C). This single copy gene was located near the centromere of chromosome XIV. It included a coding sequence of 317 amino acids that strictly...

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Bibliographic Details
Published in:The Journal of biological chemistry 1992-10, Vol.267 (30), p.21390-21395
Main Authors: Stettler, S, Mariotte, S, Riva, M, Sentenac, A, Thuriaux, P
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Blotting, Southern
CLONACION
CLONAGE
Cloning, Molecular
DNA, Fungal
DNA-directed RNA polymerase III
Fundamental and applied biological sciences. Psychology
GENE
gene mapping
GENES
Genes, Fungal
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutagenesis
NUCLEOTIDE
nucleotide sequence
NUCLEOTIDOS
prediction
Restriction Mapping
RNA Polymerase III - genetics
RPC34 gene
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
TRANSFERASAS
TRANSFERASE
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Summary:The RPC34 gene of Saccharomyces cerevisiae was cloned by immunological screening, using antibodies raised against the C34 polypeptide of the RNA polymerase III (C). This single copy gene was located near the centromere of chromosome XIV. It included a coding sequence of 317 amino acids that strictly matched two internal oligopeptides of C34. This polypeptide is a specific component of RNA polymerase III, with no significant homology to any other RNA polymerase subunit known so far. It is an essential subunit, since inactivation by deletion or nonsense mutations led to a recessive lethal phenotype. Moreover, a partially blocked mutant, rpc34-F297, had a reduced tRNA synthesis in vivo but no detectable effect on 5 S RNA synthesis. The latter phenotype was observed for all conditionally defective RNA polymerase III mutants isolated so far.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)36622-0