Effects of 2(3)- tert-butyl-4-hydroxyanisole (BHA) on in situ mitochondria of Trypanosoma cruzi

Results obtained with in situ mitochondria or Trypanosoma cruzi showed that this protozoon had only two energy coupling sites, sites II and III that correspond to higher eukaryote mitochondria. Rotenone did not inhibit the oxygen uptake or the parasite. These results suggest that the NADH-ubiquinone...

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Bibliographic Details
Published in:FEBS letters 1992-05, Vol.303 (1), p.73-76
Main Authors: Aldunate, J., Coloma-Torres, L., Spencer, P., Morello, A., Ojeda, J.M., Repetto, Y.
Format: Article
Language:English
Subjects:
2- tert-Butyl-4-hydroxyanisole (BHA)
Adenosine Diphosphate - metabolism
Animals
Butylated Hydroxyanisole - pharmacology
Coloring Agents
Mitochondria - drug effects
Mitochondria - metabolism
Mitochondrial respiratory chain
Oxidative Phosphorylation
Oxygen - metabolism
Oxygen uptake
Rhodamine 123
Rhodamines
Rotenone - pharmacology
Trypanosoma cruzi
Trypanosoma cruzi - drug effects
Trypanosoma cruzi - metabolism
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Summary:Results obtained with in situ mitochondria or Trypanosoma cruzi showed that this protozoon had only two energy coupling sites, sites II and III that correspond to higher eukaryote mitochondria. Rotenone did not inhibit the oxygen uptake or the parasite. These results suggest that the NADH-ubiquinone segment of the respiratory chain has no activity. Studies with in situ mitochondria confirmed that BHA, an antioxidant food additive, blocks the mitochondrial electron transport chain at the succinate-cytochrome b segment being the molecular basis of this trypanocidal action.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80480-5