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Meiotic and mitotic stability of transforming DNA in the phytopathogenic fungus Magnaporthe grisea

Magnaporthe grisea was transformed with cosmid pAN7-2 encoding hygromycin B resistance but containing no homology with the M. grisea genome. Rearrangement of the integrated DNA was detected in several hygromycin B-resistant progeny from cross Guy11-T10-1 (a single-copy integration site transformant)...

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Bibliographic Details
Published in:Current genetics 1992, Vol.21 (1), p.55-60
Main Authors: Tooley, P.W. (United States Dept. of Agriculture/Agricultural Research Service, Frederick, MD (USA). Foreign Disease-Weed Science Research Unit), Leung, H, Leong, S.A
Format: Article
Language:English
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Summary:Magnaporthe grisea was transformed with cosmid pAN7-2 encoding hygromycin B resistance but containing no homology with the M. grisea genome. Rearrangement of the integrated DNA was detected in several hygromycin B-resistant progeny from cross Guy11-T10-1 (a single-copy integration site transformant) x 2539 (sensitive wild-type parent), but not in hygromycin B-resistant progeny from four other crosses. Transformants produced typical lesions when inoculated onto host plants. Southern hybridization revealed rearrangements of integrated DNA in single conidial isolates of high copy transformant 2539-T1-1 re-isolated from host plants, characterized by excision of one or more copies of the transforming plasmid. Plasmid loss and rearrangement were also observed within single conidial isolates derived from transformant 2539-T1-1 following ten asexual generations on non-selective agar medium.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00318655