Enzymatic assays based on the coulometric microflow titration of ammonia and carbon dioxide

Rapidly and precisely working microflow titrations are suitable to follow enzyme catalyzed reactions producing ammonia and carbon dioxide. Ammonia and carbon dioxide were separated from the reaction zone by a gas dialysis step. Highly precise and accurate determinations of urea, glutamine, glutamate...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1992, Vol.7 (9), p.653-660
Main Authors: Fuhrmann, B., Spohn, U., Mohr, K.-H.
Format: Article
Language:English
Subjects:
Ammonia
Biological and medical sciences
Biotechnology
coulometry
Enzymatic assays
enzyme membrane
enzyme reactor
Fundamental and applied biological sciences. Psychology
gas dialysis
Methods. Procedures. Technologies
microflow titrations
Others
Various methods and equipments
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Summary:Rapidly and precisely working microflow titrations are suitable to follow enzyme catalyzed reactions producing ammonia and carbon dioxide. Ammonia and carbon dioxide were separated from the reaction zone by a gas dialysis step. Highly precise and accurate determinations of urea, glutamine, glutamate, asparagine, carbon dioxide and ammonia can be implemented. The enzymes are immobilized covalently on a preactivated membrane sandwiched with a microporous Teflon membrane. A compact and fully automated microcoulometric measuring system for fast and long term stable enzymatic determinations was developed.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(92)85023-4