Secretory expression of human alpha sub(1)-antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer

Human alpha sub(1)-antitrypsin ( alpha sub(1)-AT) was expressed and secreted in Saccharomyces cerevisiae carrying the gal1 and reg1-501 mutations under the control of GAL10 promoter. Increasing galactose from 0 to 0.5 g/l increased the extracellular concentration of alpha sub(1)-AT, though the secre...

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Bibliographic Details
Published in:Biotechnology letters 1998-03, Vol.20 (3), p.307-311
Main Authors: Chung, Bong Hyun, Kim, Su Jeong, Kang, Hyun Ah, Yu, Meyong-Hee
Format: Article
Language:English
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Summary:Human alpha sub(1)-antitrypsin ( alpha sub(1)-AT) was expressed and secreted in Saccharomyces cerevisiae carrying the gal1 and reg1-501 mutations under the control of GAL10 promoter. Increasing galactose from 0 to 0.5 g/l increased the extracellular concentration of alpha sub(1)-AT, though the secretion efficiency was decreased. The final titre of alpha sub(1)-AT secreted and the secretion efficiency were 17.9 mu g/ml and 24.8%, respectively, with 0.5 g galactose/l.
ISSN:0141-5492