Conversion of 3-demethylthiocolchicine into thiocolchicoside by Centella asiatica suspension cultures

Exogenously supplied 3-demethylthiocolchicine was converted into 3- O-glucosylthiocolchicine (thiocolchicoside) by a cell suspension culture of Centella asiatica. Around 30% of 3-demethylthiocolchicine (136 μM) was glucosylated after an 11-day incubation period. In vitro glucosylation by cell-free e...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 1998-03, Vol.47 (5), p.743-747
Main Authors: Bouhouche, N., Solet, J.M., Simon-Ramiasa, A., Bonaly, J., Cosson, L.
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
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Summary:Exogenously supplied 3-demethylthiocolchicine was converted into 3- O-glucosylthiocolchicine (thiocolchicoside) by a cell suspension culture of Centella asiatica. Around 30% of 3-demethylthiocolchicine (136 μM) was glucosylated after an 11-day incubation period. In vitro glucosylation by cell-free extracts demonstrated that the enzymatic reaction required specifically uridine diphosphate- d-glucose (UDPG1c) as a high energy glucose donor. Various endogenous phenolic compounds were assayed for their effect on the glucosyltransferase reaction. 3- O-Glucosylation of the title compound was investigated and in vitro studies were carried out with cell-free extracts.
ISSN:0031-9422
1873-3700
DOI:10.1016/S0031-9422(97)00676-6