Structure and rheological properties of a xyloglucan extracted from Hymenaea courbaril var. courbaril seeds

Hymenaea courbaril var courbaril seed xyloglucan was efficiently extracted with 0.1M NaCl, followed by ethanol precipitation (yield=72±5% w/w). Its amorphous structure was identified by the pattern of X-ray diffraction. The monosaccharide composition was determined by GC/MS analysis of the alditol a...

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Bibliographic Details
Published in:International journal of biological macromolecules 2015-02, Vol.73, p.31-38
Main Authors: Arruda, Isabel R.S., Albuquerque, Priscilla B.S., Santos, Gustavo R.C., Silva, Alexandre G., Mourão, Paulo A.S., Correia, Maria T.S., Vicente, António A., Carneiro-da-Cunha, Maria G.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Chromatography, Gel
Glucans - chemistry
Glucans - pharmacology
Hemolytic Agents - chemistry
Hemolytic Agents - pharmacology
Hymenaea - chemistry
Hymenaea courbaril
Methylation
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Plant Extracts - chemistry
Plant Extracts - pharmacology
Polysaccharide characterization
Polysaccharide structure
Rheology
Seeds - chemistry
Viscoelastic fluid
X-Ray Diffraction
Xylans - chemistry
Xylans - pharmacology
Xyloglucan
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Summary:Hymenaea courbaril var courbaril seed xyloglucan was efficiently extracted with 0.1M NaCl, followed by ethanol precipitation (yield=72±5% w/w). Its amorphous structure was identified by the pattern of X-ray diffraction. The monosaccharide composition was determined by GC/MS analysis of the alditol acetates and showed the occurrence of glucose:xylose:galactose:arabinose (40:34:20:6). One-(1D) and two-dimensional-(2D) NMR spectra confirmed a central backbone composed by 4-linked β-glucose units partially branched at position 6 with non-reducing terminal units of α-xylose or β-galactose-(1→2)-α-xylose disaccharides. The xyloglucan solution was evaluated by dynamic light scattering and presents a polydisperse and practically neutral profile, and at 0.5 and 1.0% (w/v) the solutions behave as a viscoelastic fluid. The polysaccharide did not show significant antibacterial or hemolytic activities. Overall our results indicate that xyloglucan from H. courbaril is a promising polysaccharide for food and pharmaceutical industries.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2014.11.001